Calcium Chloride Stock Solution Preparation

Buffer preparation

Calcium Chloride Stock Solution Preparation Calcium chloride is a common calcium salt used in a variety of applications. To prepare 1L of Calcium Chloride Stock Solution the following components are required. Component Amount Concentration CaCl2 (mw: 110.98 g/mol) 58 g 0.5226 M Preparation procedure Prepare 800 mL of distilled water in a suitable container. Add … Read more

Bath Solution (Ca2+/Mg2+ Free) Preparation

Buffer preparation

Bath Solution Preparation To prepare 1L of Bath Solution (Ca2+/Mg2+ Free) the following components are required; Component Amount Concentration KOH (mw: 56.11 g/mol) 7.854 g 0.14 M HEPES (mw: 238.3 g/mol) 4.766 g 0.02 M KCl (mw: 74.55 g/mol) 149 mg 0.002 M EGTA (mw: 380.35 g/mol) 1.902 g 0.005 M Methanesulfonic acid (mw: 96.11 … Read more

Pyronin Y (PY) Solution Preparation and Application

Buffer preparation

Pyronin Y(G) (PY) is a dye used for DNA staining. PY has orange spectral emission that is easily distinguishable from FITC and 7-AAD.

Nuclear Fast Red Solution Preparation and Application

Buffer preparation

This dye has been used as a counterstain in saturated aqueous potassium alum and in a counterstain solution of 0.1% (w/v) with 5% aluminum sulfate for a Prussian Blue Stain for ferric iron. It has been used as a reagent for calcium in tissue where it forms a scarlet lake.

Mayer’s Hemalum Solution Preparation and Application

Buffer preparation

This “Mayer’s hemalum solution – for microscopy” is used for human-medical cell diagnosis and serves the histological and clinico-cytological investigation of sample material of human origin.

Immunofluorescence Quenching Solution Preparation and Application

Buffer preparation

Immunofluorescence Quenching Solution is used in immunofluorescence and immunocytochemistry applications.

Cresol Red Solution Preparation and Uses

Buffer preparation

Cresol red is used as a tracking dye for DNA/RNA in agarose gel electrophoresis and protein in polyacrylamide gel electrophoresis.

Ethidium Bromide Solution Preparation and use

Buffer preparation

Ethidium bromide is used to visualize DNA in agarose gels and in many cesium chloride gradient protocols.

Chloroquine solution Preparation and Uses

Chloroquine solution Preparation and Uses

It was originally used as an antimalarial compound. It kills the erythrocytic forms of malaria parasites at all stages of development but does not affect the sporozoites.

Bradford Stock Solution Preparation

Bradford Stock Solution Preparation

This solution is mainly used to determine the concentration of proteins in solution.

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