Join Our Whatsapp and Telegram Channel to Get Free eBooks Telegram | Whatsapp

Chloroquine solution Preparation and Uses

Chloroquine solution

  • Molecular Formula of Chloroquine solution: C18H26ClN3 • 2H3PO4
  • Melting point of Chloroquine solution: 193 °C
  • It is a member of the quinoline family with multiple applications. 
  • Solutions can be sterilized by autoclaving or by filtration through a 0.2 µm membrane. Solutions of pH 4-6 are stable when heated, but are sensitive to light.

Chloroquine solution Uses

  • It was originally used as an antimalarial compound. It kills the erythrocytic forms of malaria parasites at all stages of development but does not affect the sporozoites.
  • It is also an antibiotic.4,5 It can be used at 200 mg/ml (PBS, pH 5.0) to dissociate antigenantibody complexes without denaturing red blood cell antigens.
  • A more recent usage is for DNA transfection.7,8 When used at 100 µM, it intercalates into DNA, increasing tranfection efficiency.

Required components for Chloroquine solution

The following components are required to prepare 1L of Chloroquine solution;

ComponentAmountConcentration
chloroquine diphosphate (mw: 515.86 g/mol)52 g0.1008 M
chloroquine diphosphate
chloroquine diphosphate

Chloroquine solution Preparation

  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 52 g of chloroquine diphosphate to the solution.
  3. Add distilled water until volume is 1 L.
  4. Sterilize the solution by passing it through a 0.22-µm filter; store the filtrate in foil-wrapped tubes at -20°C.

References

Related Posts

Leave a Comment

This site uses Akismet to reduce spam. Learn how your comment data is processed.

A new weapon in the battle against antibiotic resistance 16 Important Skills Needed For A Successful Career in Bioinformatics Top 5 High-Paying Biotech Jobs in India (No PhD Required) Top Emerging Trends in Bioinformatics Important Skills Needed For A Successful Career in Bioinformatics Research reveals plant pathogens repurpose phage elements for bacterial warfare Scientists show the key role of spleen and extracellular vesicles in cryptic malaria infections Scientists reveal molecular link between glucose sensing and pyroptosis cell death Scientists reconstruct ancient genomes of the two most deadly malaria parasites to identify origin and spread What are TaqMan probes?
A new weapon in the battle against antibiotic resistance 16 Important Skills Needed For A Successful Career in Bioinformatics Top 5 High-Paying Biotech Jobs in India (No PhD Required) Top Emerging Trends in Bioinformatics Important Skills Needed For A Successful Career in Bioinformatics Research reveals plant pathogens repurpose phage elements for bacterial warfare Scientists show the key role of spleen and extracellular vesicles in cryptic malaria infections Scientists reveal molecular link between glucose sensing and pyroptosis cell death Scientists reconstruct ancient genomes of the two most deadly malaria parasites to identify origin and spread What are TaqMan probes?

Adblocker detected! Please consider reading this notice.

We've detected that you are using AdBlock Plus or some other adblocking software which is preventing the page from fully loading.

We don't have any banner, Flash, animation, obnoxious sound, or popup ad. We do not implement these annoying types of ads!

We need money to operate the site, and almost all of it comes from our online advertising.

Please add biologynotesonline.com to your ad blocking whitelist or disable your adblocking software.

×