What is Pulsed-field Gel Electrophoresis (PFGE)? Principle of Pulsed Field Gel Electrophoresis (PFGE) The principle of pulsed-field gel electrophoresis (PFGE) revolves around the separation of DNA fragments based on their size using an electric field. In conventional gel electrophoresis, small DNA fragments migrate more easily through the gel matrix than large fragments. However, when dealing … Read more
Fluorescence spectroscopy is an analytical technique used to examine the characteristics and interactions of molecules. It is founded on the idea that when a molecule absorbs a photon of light and becomes excited, it emits light at a longer wavelength, a phenomenon known as fluorescence. This emitted light can be collected and studied in order … Read more
The process of collecting bacteria from the air for analysis involves several techniques, one of the simplest being the utilization of open Petri dishes filled with a growth medium. These dishes are exposed to the air, allowing airborne bacteria to settle and subsequently grow on the medium, which can then be examined to identify the … Read more
Exploring the microscopic world within soil requires precise techniques for the effective sampling of microorganisms, such as bacteria. These organisms play crucial roles in various ecological processes, including nutrient cycling, and understanding their presence and diversity is vital for both scientific research and environmental monitoring. Two widely recognized methods facilitate the direct observation of these … Read more
When examining water samples for bacterial content, direct microscopic observation often yields minimal visible bacteria due to their small size and low concentrations. To overcome this challenge and accurately assess the bacterial population in water, scientists employ the filtration technique. This method is pivotal for both quantifying bacteria per unit volume of water and analyzing … Read more
The process of collecting bacteria from the air for analysis involves several techniques, one of the simplest being the utilization of open Petri dishes filled with a growth medium. These dishes are exposed to the air, allowing airborne bacteria to settle and subsequently grow on the medium, which can then be examined to identify the … Read more
What is Satellitism Test? Definition of Satellitism Test The Satellitism Test is a biochemical assay used to differentiate Haemophilus influenzae from other Haemophilus species based on their growth factor requirements. In this test, H. influenzae grows proximally to Staphylococcus aureus colonies on blood agar due to the latter’s production of necessary growth factors, a phenomenon … Read more
Alcohol fermentation, also known as ethanol fermentation, is a sophisticated biotechnological procedure wherein specific sugars, namely glucose, fructose, and sucrose, undergo a transformation into ethyl alcohol (ethanol) and carbon dioxide. This transformation is facilitated by certain microorganisms, predominantly the yeast species Saccharomyces cerevisiae, as well as specific bacterial strains. Central to the realm of biotechnology, … Read more
What is In Vitro Fertilization (IVF)? Why IVF is done? IVF is performed for various reasons related to infertility and certain health conditions. Here are some common reasons why IVF may be done: Additionally, for individuals who do not have a functional uterus or face significant health risks during pregnancy, IVF with a gestational carrier … Read more
What is Amniocentesis? (Amniotic Fluid Test) Sample for Amniotic fluid Examination (Amniocentesis) The purpose of the test Amniocentesis, the examination of amniotic fluid, serves various purposes in prenatal diagnosis and assessment. The test provides valuable information about the fetus, allowing healthcare providers to make informed decisions regarding the health and well-being of both the mother … Read more
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