Microbiology Techniques - Biology Notes Online

Method for Balancing in Laboratory – Weight or Mass Measurement

Published on May 14, 2024 By BNO Team Fact checked & reviewed by BNO Team

The most frequent tasks in the microbiology area is the determination of the weight or mass of desired substances, chemicals. Another important aspect to be considered is the preservation of substances. Thus, the powders, as well as other granular or paste-like substances should Chamber not be placed directly on the platform for weighing of the balance. It is recommended that glazed paper or a small weigh boats is the best choice to weigh. It is recommended to use glazed papers if the material is 15 grams or less needs to be weighed. A the weigh boat pan or small beaker must be utilized if you are weighing a larger amount. Because of the the light weight of glazed papers its weight is subject to a minimal. If the weight is less of 1 gram an electronic balance is recommended. The larger amount (above 1 grams) is not to be considered using electronic balances.

Preparation Of Temporary Cotton Plugs and Permanent Cotton Plugs

Published on May 14, 2024 By BNO Team Fact checked & reviewed by BNO Team

Microorganisms are everywhere in their the distribution. In any given environment, many microorganisms are present at any given time. It is difficult to identify a specific kind of microbe until we understand their ecological needs. For instance, anaerobic microbes do not require oxygen those that are aerobic require oxygen micro-aerophiles to have oxygen however, in a small amount. So, we require cotton plugs to provide an conditions that are aerobic and keep the growing culture uninvolved with unwanted microbes. Cotton plugs are made of cotton along with air pours. Air can flow through air pours, but not the bacteria that cause microbial contamination. They are fixed to the surface of cotton fibres , and prevent them from being able from getting into flasks, tubes, etc. Air aids in for the development of microorganisms within glass equipment.

Isolation of Microorganisms from Soil

Published on May 14, 2024 By BNO Team Fact checked & reviewed by BNO Team

Soil contain different types of microorganisms which are listed below; Principle of Isolation of Microorganisms from Soil During this process, the soil sample is collected from the study site. After that, the soil sample is dissolved in distilled water and then serial dilution is performed. Then the spread-plate/streak plate method is performed on the sample … Read more

How To Use A Microscope Properly

Published on May 14, 2024 By BNO Team Fact checked & reviewed by BNO Team

Making a Wet Mount Staining a Slide (After Making a Wet Mount) Adjusting Focusing Tension There is a ring on the inside portion of the right-hand focusing knob on all other compound microscope versions (490 and above). This ring can be manually adjusted to increase or decrease tension. Changing the Eyepiece (M Series Microscope) A … Read more

How to balance a centrifuge? – Centrifuge Balancing

Published on May 14, 2024 By BNO Team Fact checked & reviewed by BNO Team

What is Centrifuge? What is Centrifuge Balancing? Why it is important to balance a centrifuge? – Why is centrifuge balanced? What Happens if a Centrifuge is Unbalanced? How Centrifuge Works? What is Centrifuge Tube? How to balance in different centrifuge? If you are utilising a portable benchtop instrument, you must ensure that the centrifuge is … Read more

Serial Dilution Method – Definition, Procedure, Application

Published on May 13, 2024 By BNO Team Fact checked & reviewed by BNO Team

What is a serial dilution? Serial dilution definition Serial dilution is a methodical process in which a solution is diluted sequentially to produce a series of progressively reduced concentrations. What is the purpose of serial dilution? – Serial Dilution Objectives Serial dilution serves as a foundational procedure in numerous scientific disciplines, especially microbiology. The primary … Read more

Rocket Immunoelectrophoresis – Definition, Principle, Procedure, Result, Application

Published on May 13, 2024 By BNO Team Fact checked & reviewed by BNO Team

Rocket Immunoelectrophoresis Principle, Procedure, Result, Application.

Rocket Immunoelectrophoresis (RIEP) also known as electro-immuno diffusion is a simple, quick and reproducible method for determining the concentration of antigen (Ag) in an unknown sample.

Coagulase Test – Principle, Procedure, Application, Result.

Published on May 13, 2024 By BNO Team Fact checked & reviewed by BNO Team

It is a type of biochemical test, which is used to differentiate Staphylococcus aureus from coagulase-negative staphylococci. S.aureus produces two forms of coagulase (i.e., bound coagulase and free coagulase).

Moist Heat Sterilization – Principle, Advantages, Disadvantages

Published on May 13, 2024 By BNO Team Fact checked & reviewed by BNO Team

Of all the options to sterilize (killing or eliminating all microorganisms, which includes the spores of bacterial growth) moist heat that is saturated steam with pressure has been the most frequently utilized and most reliable method. Moisture has more penetrating power than dry heat, and at a certain temperature, causes a greater reduction in the quantity of live microorganisms. Steam sterilization is safe cheap, low-cost, quickly microbicidal, as well as sporicidal. It quickly heats and penetrates into fabrics.

Different Aseptic Techniques in Microbiology Laboratory

Published on May 13, 2024 By BNO Team Fact checked & reviewed by BNO Team

Because the aim of biologists is to cultivate microorganisms or eukaryotic cells with no introduction of any extraneous organisms aseptic methods are essential for ensuring that experiments are conducted with precision and accuracy. Always keep in mind that a totally safe working environment for scientists does not exist.