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Affinity chromatography – Principle, Types, Steps, Applications

Affinity chromatography Principle, Types, Steps, Applications

Affinity chromatography is a type of liquid chromatography technique which is used for separation and purification of biomolecules based on a highly specific and reversible interaction between the target molecule and a specific ligand. It is the process where the target molecule selectively binds with a ligand that is immobilized on a solid matrix. This … Read more

Bench Top Centrifuge – Principle, Procedure, Types, Parts, Uses

Bench Top Centrifuge - Principle, Types, Parts, Uses

A bench top centrifuge is a laboratory instrument which is used for separation of components present in a liquid sample based on their density. It is a compact device that is designed to be placed on laboratory bench and is commonly used in routine laboratory work. It is the process in which centrifugal force is … Read more

Hematocrit Centrifuge -Principle, Procedure, Parts, Uses

Hematocrit Centrifuge - Definition, Principle, Parts, Uses

A hematocrit centrifuge is a laboratory instrument which is used to determine the hematocrit value of blood. It is the percentage volume of red blood cells present in the total volume of whole blood and this value is also referred to as packed cell volume (PCV). It is commonly used in hematology laboratories for routine … Read more

Native Polyacrylamide Gel Electrophoresis (PAGE)

Native Polyacrylamide Gel Electrophoresis (PAGE)

Native PAGE is the method where proteins are separated in their original folded condition without any use of denaturing reagents. It is the process in which the movement of proteins in a polyacrylamide gel is controlled by the natural charge, size and the native conformation of the molecules. The proteins migrate through the gel matrix … Read more

Bioreactor – Types, Design, Parts, Applications, Limitations

Bioreactor - Types, Design, Parts, Applications, Limitations

A bioreactor is the vessel in which biological reactions is carried out in controlled conditions. It is the process where microorganisms, plant cells or animal cells are allowed to grow and produce useful products. It is designed as a closed container in which temperature, pH, dissolved oxygen (DO) and nutrient level is maintained with the … Read more

Microtome – Principle, Parts, Types, and Uses

Microtome - Principle, Parts, Types, and Uses

What is Microtome? A microtome is a laboratory device that is used for cutting very thin sections of different materials for microscopic examination. It is the instrument by which uniform slices are produced, and it is the process that helps the light or the electron to pass through the specimen so that the internal structures … Read more

Spectrophotometer Cuvette – Features, Types, Materials, Precautions, Advantages

Spectrophotometer Cuvette - Features, Types, Materials, Precautions, Advantages

A spectrophotometer cuvette is a small optical cell that is used to hold the liquid sample during absorbance or transmittance measurement. It is usually made in a rectangular shape and two opposite sides are kept clear and highly polished so that the light beam can pass through the solution without scattering. It is the process … Read more

How to Calibrate ph Meter? Step By step procedure and requirement

How to Calibrate ph Meter?

A pH meter is an instrument that is used to measure the pH of a solution, and it is the value that shows how acidic or alkaline the solution is. It is the process where a glass electrode and a reference electrode are placed in the sample, and the potential difference formed between them is … Read more

Gradient PCR – Definition, Principle, Process, Functions

Gradient PCR - Definition, Principle, Process, Functions

Gradient PCR is referred to as a PCR optimization method where a range of annealing temperatures is tested at the same time in a single run. It is done in a thermal cycler that contain a gradient block, and this block is made in such way that one side hold lower temperature while the opposite … Read more

Bright Field Microscope – Definition, Parts, Working Principle, Application

Bright Field Microscope - Definition, Parts, Working Principle, Application

What is Bright Field Microscope? Bright field microscope is an optical microscope that produce a dark image of the specimen against a bright background, and it is the most common compound light microscope used in biological laboratories. It is the system where the specimen is illuminated from below by a uniform beam of light, and … Read more

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