Cultivation of bacteria

Cultivation of bacteria

Bacteria constitute a vital element in the ecology. They are essential to our health as well as the environmental health, play an essential role in the production of food, and offer bioengineers the tools to harness their abilities and produce compounds. However, they could also be harmful, causing harm and diseases. The capacity to cultivate

Colony Morphology of Bacteria with their identification and Examples

Colony Morphology of Bacteria and Examples

What is Colony Morphology of Bacteria? Colony morphology of bacteria means the visible features seen in bacterial colonies when they grow on solid media like agar plates. Each colony usually comes from one bacterial cell that divides and forms a clump. These clumps or colonies can look very different based on the species and conditions,

Clostridium tetani – Overview

Clostridium tetani - Overview

Tetanus is caused by C. tetani, an obligate anaerobic Gram-positive bacillus. Tetanus is an infectious disease characterised by increased muscle tone and spasms caused by the release of tetanospasmin, a neurotoxin produced by C. tetani when it is inoculated into humans. Morphology of Clostridium tetani C. tetani demonstrates the following characteristics: Geographical distribution of Clostridium

Clostridium botulinum – Overview

Clostridium botulinum - Overview

Types of Botulism  There are three primary forms of botulism: baby, wound, and foodborne. Additional clinical classifications include adult intestinal toxaemia and iatrogenic botulism. 1. Foodborne botulism 2. Infant botulism 3. Wound botulism 4. Adult intestinal toxemi 5. Iatrogenic botulism Morphology of Clostridium botulinum C. botulinum has the following characteristics: Habitat of Clostridium botulinum Geographical

Lipopolysaccharide (LPS) – Structure, Functions

Lipopolysaccharide (LPS) Structure, Functions

Structure and Composition of Lipopolysaccharide (LPS) It is composed of three components like; O-antigen Core Structure of Core or Core oligosaccharide (or Core-OS) The core domain always contains an oligosaccharide component which attaches directly to lipid A and commonly contains sugars such as heptose and 3-deoxy-D-mannooctulosonic acid (also known as KDO or keto-deoxyoctulosonate). It is

Sphaeroplasts – Definition, Formation, Applications

Sphaeroplasts - Definition, Formation, Applications

What is Sphaeroplast? A Spheroplast (or sphaeroplast according to British use) can be described as a microbe organism with a cell wall that is almost entirely gone through the penicillin or Lysozyme. According to certain definitions the term is employed to refer to Gram-negative bacteria. In other definitions, the word also includes yeasts. Spheroplast’s name

Protoplasts – Definition, Application and Protoplasts Culture

Protoplasts - Definition, Application and Protoplasts Culture

Protoplast Definition Protoplasts are plant cells that are completely naked with no cell wall however they do have plasma membranes and other components of the cell. They are functional cells, but without the presence of a barrier, cell wall. Protoplasts from various species can be merged to create a hybrid, and this is known as

Protoplasts Isolation

Protoplasts Isolation

The protoplast, sometimes referred to as a naked plant cell refers to all the constituents in a cell of plant that are not the wall of the cell. The term”protoplast” was first used in the work of Hanstein in 1880 to refer to the living matter contained within the membrane of the plant cell. The

Protoplast Culture Methods

Protoplast Culture Methods

Isolated protoplasts can be cultured in a liquid medium , or semisolid agar medium , either in thin layers or tiny drops of nutrient medium in petridish. The medium used to cultivate protoplasts is the same as that needed for suspension or callus culture. The increase in calcium concentration aids in maintaining the membrane’s integrity.