Gene Cloning – Steps, Definition, Applications

Gene Cloning - Steps, Definition, Applications

What is Gene cloning? “DNA Cloning” is a molecular biology technique that is utilized to create exact copies, or clones, of one particular gene or DN.” History of Gene cloning Principle of Gene Cloning Requirements for Gene Cloning Restriction Enzyme Digestion and Ligation Enzyme Source Recognition Sequence Restriction Digest EcoRI Escherichia coli 5’GAATTC3’CTTAAG   5′—G↓AATTC—3’3′—CTTAA↑G—5′ … Read more

DNA polymerase IV – Definition, Structure, Mechanism, Functions

DNA polymerase IV - Definition, Structure, Mechanism, Functions

What is DNA polymerase IV? Definition of DNA polymerase IV DNA polymerase IV is a prokaryotic enzyme encoded by the dinB gene, involved in mutagenesis and DNA repair processes, especially during the SOS response to DNA damage. Unlike many polymerases, it lacks proofreading activity, making it error-prone, but it plays a crucial role in translesion … Read more

Deletion Mutation – Definition, Causes, Mechanism, Examples

Deletion Mutation - Definition, Causes, Mechanism, Examples

What is Deletion Mutation? Definition of Deletion Mutation A deletion mutation is a genetic anomaly in which a segment of a chromosome or DNA sequence is omitted during DNA replication, leading to the absence of specific nucleotides or entire chromosomal segments. This can result in altered gene function or expression. Causes of Deletion Mutation Deletion … Read more

Watson and Crick DNA Model

Watson and Crick DNA Model

In 1953, James Watson and Francis Crick made a groundbreaking contribution to the understanding of DNA structure. By combining physical and chemical data, they proposed a model for DNA as a double helix, a twisted molecule consisting of two complementary strands held together by hydrogen bonds. This model revolutionized our knowledge of genetics and unlocked … Read more

Nucleoside – Definition, Types, Structure, Functions

Nucleoside - Definition, Types, Structure, Functions

What is Nucleoside? A nucleoside is a molecule composed of a pentose sugar linked to a nitrogenous base or glycosylamine. It can be considered as a nucleotide without a phosphate group. Nucleosides are essential components of DNA and RNA, playing a vital role in the storage and transmission of genetic information. In DNA, the nucleosides … Read more

miRNA (microRNA) Structure, Functions, Application

miRNA (microRNA) Structure, Functions, Application

Micro-RNAs (miRNAs) are a family of non-coding RNAs (ncRNAs) that regulate gene expression post-transcriptionally. Since their discovery in 1993, they have been the topic of extensive research due to their role in numerous crucial biological processes. Unlike other noncoding RNAs, microRNAs are created from transcriptional units that are processed by a particular group of endonucleases. … Read more

Frederick Griffith Experiment – Bacterial transformation

Frederick Griffith Experiment: Bacterial transformation

Griffith Experiment & Transforming Principle Frederick Griffith, a British bacteriologist, conducted a series of studies with Streptococcus pneumoniae bacteria and mice in 1928. Griffith was not attempting to detect genetic material; rather, he was attempting to produce a vaccine for pneumonia. Griffith utilised two strains of bacteria identified as R and S in his tests. … Read more

Pyrimidines and Purines Nucleotides – Structure, Occurrence and Properties

Pyrimidines and Purines Nucleotides - Structure, Occurrence and Properties

Pyrimidines and Pyrimidine Nucleotides Structure of Pyrimidines and Pyrimidine Nucleotides Occurrence of Pyrimidines and Pyrimidine Nucleotides Properties of Pyrimidines and Pyrimidine Nucleotides Biosynthesis of Pyrimidine  Pyrimidine Nucleotide Examples Cytosine Thymine Uracil Purines and Purine Nucleotides Structure of Purines and Purine Nucleotides Occurrence of Purines and Purine Nucleotides Properties of Purines and Purine Nucleotides Purine Nucleotides … Read more

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