Southern Blot – Definition, Principle, Steps, Importance
Southern Blotting is a molecular biology technique used for the identification of a specific DNA sequence in a collected DNA sample.
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Widal Test – Principle, Procedure, Result, Advantages, Limitation
The Widal test is an agglutination test that determines any antibodies present in the patient’s serum that are generated against the causal agents of the enteric febrile illness (Salmonella Typhi, and Salmonella paratyphi B, A as well as C).
Counter Current Immunoelectrophoresis – Principle, Procedure, Result.
Counter current immunoelectrophoresis is a modification of immunoelectrophoresis in which antigen and antibody migrate towards opposite directions and form a visible white precipitate in the area between the wells.
Serial Dilution Method – Definition, Procedure, Application
What is a serial dilution? Serial dilution definition Serial dilution is a methodical process in which a solution is diluted sequentially to produce a series of progressively reduced concentrations. What is the purpose of serial dilution? – Serial Dilution Objectives Serial dilution serves as a foundational procedure in numerous scientific disciplines, especially microbiology. The primary … Read more
Rocket Immunoelectrophoresis – Definition, Principle, Procedure, Result, Application
Rocket Immunoelectrophoresis (RIEP) also known as electro-immuno diffusion is a simple, quick and reproducible method for determining the concentration of antigen (Ag) in an unknown sample.
Coagulase Test – Principle, Procedure, Application, Result.
It is a type of biochemical test, which is used to differentiate Staphylococcus aureus from coagulase-negative staphylococci. S.aureus produces two forms of coagulase (i.e., bound coagulase and free coagulase).
Spread Plate Method – Procedure, Principle, Purpose, Result.
Spread Plate Method in microbiology The spread plate method is used to isolate individual colonies from a diluted sample of mix population. Different methods have been developed for the isolation of microorganisms from a mixture sample but among them Spread plate culture method is the most widely used technique in laboratory. An ideal spread plate … Read more
Pour Plate Method – Principle, Procedure, Objective, Result, Advantages
The pour Plate Method technique was created in the laboratory by Robert Koch and is still widely used in the laboratory since his time. This technique is appropriate for Microaerophilic, facultative, or anaerobic microorganisms. It is straightforward to use, requires fewer resources, and is simple and cost-effective. However, it requires the specimen to be placed … Read more
Streak Plate Method – Patterns, Procedure, Principle
Streak Plate Method Overview There are two types of microbial culture such as mixed culture and pure culture. The mixed culture contains two or more species while the pure culture contains only a single species. During the identification of a microorganism, the first and important step is to isolate the individual species from a mixed … Read more
Blood Cross Matching – Principle, Procedure, Result.
Cross Match is a laboratory test, performed prior to a blood transfusion to determine whether donor blood is compatible (or incompatible) with recipient blood.