Lactobacilli MRS Broth and Agar – Composition, Preparation, Principle

Lactobacilli MRS Broth and Agar - Composition, Preparation, Principle

MRS Broth was developed by deMan, and co. in order to facilitate the most abundant growth of lactobacilli derived from feces, oral and other samples. Lactobacilli MRS Broth is a better media for lactobacilli. It allows for healthy growth and is especially suitable for several very discerning strains that grow poorly in other media.

Pyronin Y (PY) Solution Preparation and Application

Buffer preparation

Pyronin Y(G) (PY) is a dye used for DNA staining. PY has orange spectral emission that is easily distinguishable from FITC and 7-AAD.

Nuclear Fast Red Solution Preparation and Application

Buffer preparation

This dye has been used as a counterstain in saturated aqueous potassium alum and in a counterstain solution of 0.1% (w/v) with 5% aluminum sulfate for a Prussian Blue Stain for ferric iron. It has been used as a reagent for calcium in tissue where it forms a scarlet lake.

Mayer’s Hemalum Solution Preparation and Application

Buffer preparation

This “Mayer’s hemalum solution – for microscopy” is used for human-medical cell diagnosis and serves the histological and clinico-cytological investigation of sample material of human origin.

Immunofluorescence Quenching Solution Preparation and Application

Buffer preparation

Immunofluorescence Quenching Solution is used in immunofluorescence and immunocytochemistry applications.

Chloroquine solution Preparation and Uses

Chloroquine solution Preparation and Uses

It was originally used as an antimalarial compound. It kills the erythrocytic forms of malaria parasites at all stages of development but does not affect the sporozoites.

Bradford Stock Solution Preparation

Bradford Stock Solution Preparation

This solution is mainly used to determine the concentration of proteins in solution.

5-Fluorocytosine (5-FC) Solution Preparation

5-Fluorocytosine (5-FC) Solution Preparation

5-fluorocytosine (5-FC) also known as Flucytosine. 5-Fluorocytosinewas first synthesized in 1957 as anti-tumor drug. It is an antifungal medication which is used with amphotericin B against Candida infections and cryptococcosis. This drug is mainly taken by mouth and by injection into a vein.

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