Endospore Staining – Principle, Procedure, Result
There are two methods which are commonly used to stain bacterial endospore such as Schaeffer Fulton and Klein methods.
Staining
In microbiology, staining refers to the laboratory technique of applying dyes (stains) to microorganisms or tissue/cell samples so that they become more visible under the microscope.
Because most bacteria and cells are naturally transparent, they cannot be seen clearly in bright-field microscopy. Staining solves this problem by adding artificial color, which increases contrast, visibility, and differentiation.
Simple meaning
Staining means coloring microorganisms with a dye so that their shape, size, and structural details can be observed.
Toluidine Blue Staining – Principle, Procedure, Uses
Toluidine blue is a basic thiazine metachromatic dye that stains nuclei blue, and can be used to differentiate different types of granules (e.g. within mast cells).
Iron-Hematoxylin Staining – Principle, Procedure, Uses
Hematoxylin is extracted from a leguminous plant, known as Haematoxylon campechianum, thus it is a natural dye. It forms hematein by oxidation (Ripening).
Acridine Orange Staining – Principle, Procedure, Uses
Acridine Orange contains metachromatic properties, that’s why it is employed in fluorescence microscopy and flow cytometry study of cellular physiology and cell cycle state, including the fluorescent microscopic examination of microorganisms.
Collagen Hybridizing Peptide Staining – Principle, Procedure, Uses
Collagen Hybridizing Peptide stain is a vital stain used for the detection of collagen degraded tissues, mainly used in Developmental Biology, Histology, and Histopathology.
Kinyoun stain (Acid Fast Cold) Method – Principle, Procedure, Result
Kinyoun method also known as Kinyoun stain (cold method), was first developed by Joseph J. Kinyoun.
Ziehl-Neelsen Staining (ZN Staining) – Principle and Procedure, Result
Ziehl Neelsen stinging method is used to differentiate between the acid-fast and non-acid fast bacteria.
Lactophenol Cotton Blue (LPCB) Staining – Principle, Procedure, Result.
Lactophenol Cotton Blue (LPCB) staining is the simple wet-mount method used for microscopic examination of fungi, and it is considered one of the common procedure in mycology. It is the process where fixation, staining, and preservation of fungal structures is achieved within a single reagent. The solution is prepared by phenol, lactic acid, glycerol and … Read more
Sudan Black B Staining – Principle, Procedure, Uses
Sudan Black is a slightly basic dye and will combine with acidic groups in compound lipids, thus staining phospholipids also. An alternative stain to the Sudan Black B stain.
Parasporal Crystal Stain – Principle, Procedure, Result
Production of parasporal crystals is a unique ability (among Bacillus species) of Bacillus thuringiensis. This stain is a means of rapid identification of the species.