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Giemsa Stain- Principle, Procedure, Results, Uses

Procedure of Giemsa staining

Giemsa stain is a polychromatic nucleic acid stain that is used mainly for demonstrating different blood cells and parasites in stained blood films. It is named after the German chemist Gustav Giemsa. It belongs to a group of stains known as Romanowsky stains. These are neutral stains made from a mixture of oxidized methylene blue, … Read more

Capsule Staining – Procedure, Principle, Result

Maneval's capsule staining

Capsule staining is a differential staining method, where two different stains are used such as the primary stain and the counterstain.

Endospore Staining – Principle, Procedure, Result

Endospore Staining Principle, Procedure, Result

There are two methods which are commonly used to stain bacterial endospore such as Schaeffer Fulton and Klein methods.

Toluidine Blue Staining – Principle, Procedure, Uses

Toluidine blue stain of Mast cell

Toluidine blue is a basic thiazine metachromatic dye that stains nuclei blue, and can be used to differentiate different types of granules (e.g. within mast cells).

Iron-Hematoxylin Staining – Principle, Procedure, Uses

Iron-Hematoxylin Staining

Hematoxylin is extracted from a leguminous plant, known as Haematoxylon campechianum, thus it is a natural dye. It forms hematein by oxidation (Ripening).

Acridine Orange Staining – Principle, Procedure, Uses

Acridine Orange Staining

Acridine Orange contains metachromatic properties, that’s why it is employed in fluorescence microscopy and flow cytometry study of cellular physiology and cell cycle state, including the fluorescent microscopic examination of microorganisms.

Collagen Hybridizing Peptide Staining – Principle, Procedure, Uses

Collagen Hybridizing Peptide Staining

Collagen Hybridizing Peptide stain is a vital stain used for the detection of collagen degraded tissues, mainly used in Developmental Biology, Histology, and Histopathology.

Kinyoun stain (Acid Fast Cold) Method – Principle, Procedure, Result

Kinyoun stain (Acid Fast Cold) Method: Principle, Procedure, Result

Kinyoun method also known as Kinyoun stain (cold method), was first developed by Joseph J. Kinyoun.

Ziehl-Neelsen Staining (ZN Staining) – Principle and Procedure, Result

Result of Ziehl-Neelsen Staining or acid-fast staining

Ziehl Neelsen stinging method is used to differentiate between the acid-fast and non-acid fast bacteria.

Lactophenol Cotton Blue (LPCB) Staining – Principle, Procedure, Result.

Lactophenol Cotton Blue Staining of aspergillus

Lactophenol Cotton Blue (LPCB) staining is the simple wet-mount method used for microscopic examination of fungi, and it is considered one of the common procedure in mycology. It is the process where fixation, staining, and preservation of fungal structures is achieved within a single reagent. The solution is prepared by phenol, lactic acid, glycerol and … Read more

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