Papanicolaou Staining (Pap stain) – Principle, Procedure, Results, Applications
Papanicolaou stain can also be referred to as the pap stain, and the process that causes the stain is referred to as a pap smear.
Staining
Albert Stain – Principle, Procedure, Result, Uses
Different stains have been developed over time to distinguish bacteria species, separating them morphologically and the specific characteristics they possess. The most popular stain is Gram staining, acid-fast staining, and endospore staining. Each stain aims at identifying and defining bacteria according to their forms and morphologies.
Types of Stains used in Microbiology
Different stains react or concentrate on different areas of a tissue or cell These properties can be utilized to highlight certain areas or regions. A few of the most well-known biological staining methods can be found below. If not otherwise indicated All of these dyes can be used on tissues and cells that are fixed as well as essential dyes (suitable for use in live organisms) are indicated.
Types of Staining Techniques With Examples and Uses
Staining is a method used to boost the contrast of samples, usually at the microscopic scale.
Wright Giemsa Stain – Protocol, Principle, Result
This stain was discovered by German chemist Gustav Giemsa, that’s why its called Wright Giemsa Stain.
Cell Membrane Staining – Principle, Procedure, Result, Uses
Cell membrane staining is the process in which the plasma membrane of a cell is visualized with the help of dyes or fluorescent probes. It is the thin outer covering of the cell, and it is normally transparent, so it cannot be seen clearly under a simple light microscope. It is the process where special … Read more
Nuclear Staining- Principle, Procedure, Uses
Nuclear staining is the process in which the nucleus of a cell is given artificial colour so that it becomes visible under microscope. It is the main method used in histology and cytology because the nucleus is naturally transparent and it needs specific dyes for proper contrast. The nucleus contains DNA and chromatin materials which … Read more
Cell Wall Staining by Dayr’s Method – Principle, Procedure, Uses
The composition of cell wall varies from species to species, it has been reported that the main constituents of cell wall is chitin, hemicellulose, and cellulose. In addition, prokaryotic cell wall contains peptidoglycan (also known as murein and mucopeptide). Peptidoglycan is mainly composed of sugar, amino acids (peptide; amino acids + glycan; sugar).
Cell Viability test using Propidium Iodide (PI) – Principle, Procedure, uses
Cell viability analysis using Propidium Iodide is a simple method in which the dye is used to distinguish between living and dead cells. It is the process where Propidium Iodide (PI) is added to a cell suspension and the stained cells is then examined usually by flow cytometer. PI is a red-fluorescent dye that binds … Read more
Viability Staining by Loeffler’s Method – Principle, Procedure, Uses
Loeffler’s methylene blue staining is a simple staining method used mainly for observing the morphology of Corynebacterium diphtheriae. It is the process where Loeffler’s alkaline methylene blue solution is applied on a fixed smear, and this stain contains a small amount of potassium hydroxide (KOH) which makes the solution alkaline. It is the alkalinity that … Read more