Agarose gel electrophoresis (AGE) is an approach that is used to distinguish DNA from RNA based on their molecular sizes.
The separation of RNA and DNA molecules is accomplished when nucleic acids that are negatively charged travel through an agarose structure under an influence of an electrical fields (electrophoresis).
Despite all the technical advances in scientific laboratories, the Neubauer Chamber is still the most widely used method for cell counting worldwide. This technical data sheet was created to assist experienced and novice researchers in performing a cell count using a Neubauer or Hemocytometer. These principles can be applied to any cell-counting chamber. However, the … Read more
MacConkey Agar is a selective and differentiated culture medium widely used to isolate of Gram-negative enteric bacteria.
It is selective since it allows for the growth of gram-negative bacteria , and differentiating the bacteria that are gram-negative based on their metabolic process of lactose.
A KOH pre-test is a quick, non-invasive procedure to diagnose fungal infections that affect the nails or skin.
The cells are collected from the affected region and put on a slide using an acid composed of potassium hydroxide. They are looked at under a microscope in order to determine if there are evidence of the presence of a mold.
Bile Esculin test The Bile Esculin test is a biological test that is used to distinguish Enterococci from Group D Streptococci from non-group D viridans group Streptococci by their capacity to hydrolyze esculin. A variety of organisms are capable hydrolyzing esculin. But only a handful of them can do it when they are surrounded by … Read more
Two different methods of blood tests, thin and thick microscopy, are considered to be the “gold standard” for the diagnosis of malaria. A Quantitative Buffy Coat(QBC) is an additional quick and direct test to make the identification of malaria.
The Arneth Count determines the percentage distribution of various types of neutrophils based on their nuclear lobes. It isn’t used often in modern medicine. Joseph Arneth divided neutrophils into five types. Stage Nuclear lobes Diagram Normal Range Stage 1 (N1) Nucleus is unilobed.The nucleus is C or U shaped. 2-5% Stage 2 (N2) Nucleus is … Read more
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