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Okazaki Fragments – Definition, Discovery, Formation, Enzymes, Importance

Okazaki Fragments - Definition, Discovery, Formation, Enzymes, Importance

Okazaki fragment is the short segment of DNA that is synthesized on the lagging strand during DNA replication. It is formed because the DNA polymerase can extend the new strand only in the 5’–3’ direction, so the strand that is oriented in the 3’–5’ direction is produced in a discontinuous manner. It is initiated by … Read more

Post-transcriptional Modification – Definition, Types, Process, Importance

Post-transcriptional modification

Post-transcriptional modification is a set of biological processes common to most eukaryotic cells by which an primary RNA transcript is chemically altered following transcription from a gene to produce a mature, functional RNA molecule that can then leave the nucleus and perform any of a variety of different functions in the cell.

Different model of Replication – Theta, rolling circle, and linear DNA replication

Various model of Replication - Theta, rolling circle, and linear DNA replication

DNA replication is the process by which genetic material is copied before cell division happens, it’s considered essential for inheritance. Among different models, the main ones observed are Theta model, Rolling Circle model, and Linear DNA replication. Each model are used by different organisms or DNA types (like plasmids, viruses, or eukaryotic chromosomes). The three … Read more

Real-Time PCR (qPCR) – Definition, Principle, Protocol, Application, Advantages

Real-Time PCR Principle, Protocol, Application, Advantages.

A real-time polymerase chain reaction (real-time PCR) is a conventional polymerase chain reaction (PCR) based laboratory technique used in molecular biology for real-time monitoring of the amplification of a targeted DNA molecule during the PCR, not at its end, as the conventional PCR do.

Electroporation – Principle, Steps, Applications

Yeast Transformation by Electroporation

What is Electroporation? Electroporation is a fascinating technique that temporarily makes cell membranes more permeable by applying brief electric pulses. This method, widely used in labs and medical settings, allows substances like DNA, drugs, or proteins to enter cells by creating tiny pores in the membrane. Imagine zapping cells with a controlled jolt of electricity—this … Read more

What is Semiconductor Sequencing? – Principle, procedure, uses

Semiconductor sequencing flow diagram

What is Semiconductor Sequencing? Semiconductor sequencing is the integration of microchip technology with DNA sequencing where electrical signals replace the detection of light. This sequencing method is unique because it uses complementary metal-oxide semiconductor chips associated with sequencing by synthesis chemistry; so, in this respect, it opens up opportunities to make DNA analysis quicker and … Read more

Theta Model of Replication – Steps, Applications, Examples

Theta Model of Replication - Definition, Mechanism

What is Theta model of DNA Replication? The theta model of DNA replication is a mechanism for DNA replication which occurs in circular DNA molecules, in particular, those of bacteria, and also the mitochondria and chloroplasts. True to its name, this replication process creates an intermediate structure during replication that resembles the Greek letter theta … Read more

Telomerase – Structure, Mechanism, Regulation, Functions

Telomeres and Telomerase

What is Telomerase? Telomerase (terminal transferase) is a ribonucleoprotein enzyme that extends telomeres, the repetitive nucleotide sequences at the ends of eukaryotic chromosomes. Telomeres function as protective caps, stopping chromosome ends from being erroneously perceived as DNA damage or from bonding with neighboring chromosomes. Most eukaryotes use telomerase to maintain their telomeres, but some organisms, … Read more

Telomeres – Structure, Aging, Shortening, Functions

Telomeres and Telomerase

What Are Telomeres (telomere)? At the ends of linear eukaryotic chromosomes are specialized structures called telomeres. DNA degradation is prevented by repetitive nucleotide sequences of the telomere, which caps and so stabilizes chromosomal DNA during cell division. The termini of chromosomes not only have no translation product but instead have a highly important role in … Read more

What is DNA Replication? – Steps, Enzymes, Mechanism, Applications

DNA Replication Mechanism, Definition, Requirements, Steps

What is DNA Replication? Definition of DNA Replication DNA replication is the biological process by which a cell duplicates its DNA molecule, producing two identical copies from one original DNA strand, ensuring the transmission of genetic information during cell division. DNA Replication Enzymes and Proteins DNA replication is a fundamental biological process, relying on various … Read more

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