Which one or more of the following statements correctly describe(s) fluorescence spectroscopy?

(A) The emission maxima (λmax) is independent of the excitation wavelength.
(B) The emission maxima (λmax) depends on the concentration of a quencher.
(C) The emission maxima (λmax) varies with solvent polarity.
(D) The emission maxima (λmax) varies with temperature.

SouravAugust 18, 2024

Answer

Answer: (C) The emission maxima (λmax) varies with solvent polarity.
(D) The emission maxima (λmax) varies with temperature.

Explanation:
The emission maxima in fluorescence can shift due to changes in the environment around the fluorophore, such as solvent polarity and temperature, as these factors can alter the energy levels and thus the wavelength of emitted light.

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