How to prepare buffer using the Henderson-Hasselbalch equation?
How to prepare buffer using the Henderson-Hasselbalch equation?
Cite this post:
Sourav Pan. (2025, April 24). How to prepare buffer using the Henderson-Hasselbalch equation?. Biology Notes Online. Retrieved from https://biologynotesonline.com/qa/how-to-prepare-buffer-using-the-henderson-hasselbalch-equation/
Sourav Pan. "How to prepare buffer using the Henderson-Hasselbalch equation?." Biology Notes Online, 24 April 2025, biologynotesonline.com/qa/how-to-prepare-buffer-using-the-henderson-hasselbalch-equation/.
Sourav Pan. "How to prepare buffer using the Henderson-Hasselbalch equation?." Biology Notes Online (blog). April 24, 2025. https://biologynotesonline.com/qa/how-to-prepare-buffer-using-the-henderson-hasselbalch-equation/.
Answered
1. Choose a weak acid with a pKa close to the desired pH. 2. Use the equation to calculate the required ratio of [A⁻]/[HA]. 3. Determine the total concentration needed for buffering capacity. 4. Calculate the individual concentrations of A⁻ and HA using the ratio and total concentration. 5. Prepare the solution by mixing the appropriate amounts of acid and conjugate base.
Cite this post:
Sourav Pan. (2025, April 24). How to prepare buffer using the Henderson-Hasselbalch equation?. Biology Notes Online. Retrieved from https://biologynotesonline.com/qa/how-to-prepare-buffer-using-the-henderson-hasselbalch-equation/
Sourav Pan. "How to prepare buffer using the Henderson-Hasselbalch equation?." Biology Notes Online, 24 April 2025, biologynotesonline.com/qa/how-to-prepare-buffer-using-the-henderson-hasselbalch-equation/.
Sourav Pan. "How to prepare buffer using the Henderson-Hasselbalch equation?." Biology Notes Online (blog). April 24, 2025. https://biologynotesonline.com/qa/how-to-prepare-buffer-using-the-henderson-hasselbalch-equation/.
