Biochemistry 2 Views 1 Answers

Sourav PanApril 24, 2025

How to use the Henderson-Hasselbalch equation for pH indicators?

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Sourav Pan. (2025, April 24). How to use the Henderson-Hasselbalch equation for pH indicators?. Biology Notes Online. Retrieved from https://biologynotesonline.com/qa/how-to-use-the-henderson-hasselbalch-equation-for-ph-indicators/

Sourav Pan. "How to use the Henderson-Hasselbalch equation for pH indicators?." Biology Notes Online, 24 April 2025, biologynotesonline.com/qa/how-to-use-the-henderson-hasselbalch-equation-for-ph-indicators/.

Sourav Pan. "How to use the Henderson-Hasselbalch equation for pH indicators?." Biology Notes Online (blog). April 24, 2025. https://biologynotesonline.com/qa/how-to-use-the-henderson-hasselbalch-equation-for-ph-indicators/.

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Sourav PanApril 24, 2025

Answered

Indicators change color over a specific pH range. Use the equation to determine the pH at which the ratio of the deprotonated to protonated form of the indicator is 1, i.e., when pH = pKa of the indicator. This is the midpoint of the color change range.

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