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Sourav PanApril 24, 2025

How to use the Henderson-Hasselbalch equation with unknown volume?

How to use the Henderson-Hasselbalch equation with unknown volume?

Cite this post:

Sourav Pan. (2025, April 24). How to use the Henderson-Hasselbalch equation with unknown volume?. Biology Notes Online. Retrieved from https://biologynotesonline.com/qa/how-to-use-the-henderson-hasselbalch-equation-with-unknown-volume/

Sourav Pan. "How to use the Henderson-Hasselbalch equation with unknown volume?." Biology Notes Online, 24 April 2025, biologynotesonline.com/qa/how-to-use-the-henderson-hasselbalch-equation-with-unknown-volume/.

Sourav Pan. "How to use the Henderson-Hasselbalch equation with unknown volume?." Biology Notes Online (blog). April 24, 2025. https://biologynotesonline.com/qa/how-to-use-the-henderson-hasselbalch-equation-with-unknown-volume/.

Sourav Pan
Sourav PanApril 24, 2025

Answered

If the volume is unknown but constant for both components, use the ratio of moles instead of concentrations: pH = pKa + log10(nA⁻/nHA), where nA⁻ and nHA are the moles of conjugate base and acid, respectively.

Cite this post:

Sourav Pan. (2025, April 24). How to use the Henderson-Hasselbalch equation with unknown volume?. Biology Notes Online. Retrieved from https://biologynotesonline.com/qa/how-to-use-the-henderson-hasselbalch-equation-with-unknown-volume/

Sourav Pan. "How to use the Henderson-Hasselbalch equation with unknown volume?." Biology Notes Online, 24 April 2025, biologynotesonline.com/qa/how-to-use-the-henderson-hasselbalch-equation-with-unknown-volume/.

Sourav Pan. "How to use the Henderson-Hasselbalch equation with unknown volume?." Biology Notes Online (blog). April 24, 2025. https://biologynotesonline.com/qa/how-to-use-the-henderson-hasselbalch-equation-with-unknown-volume/.

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