Determine the correctness or otherwise of the following Assertion (a) and the Reason (r) Assertion: MTT assay is used to determine cell viability based on the principle of colour formation by DNA fragmentation. Reason: MTT assay is used to determine cell viability based on the colour development by converting tetrazolium soluble salt to insoluble salt. (A) both (a) and (r) are true and (r) is the correct reason for (a) (B) both (a) and (r) are true and (r) is not the correct reason for (a) (C) (a) is true but (r) is false (D) (a) is false but (r) is true
Determine the correctness or otherwise of the following Assertion (a) and the Reason (r)
Assertion: MTT assay is used to determine cell viability based on the principle of colour formation by DNA fragmentation.
Reason: MTT assay is used to determine cell viability based on the colour development by converting tetrazolium soluble salt to insoluble salt.
(A) both (a) and (r) are true and (r) is the correct reason for (a)
(B) both (a) and (r) are true and (r) is not the correct reason for (a)
(C) (a) is true but (r) is false
(D) (a) is false but (r) is true
Answer
Answer: (D) (a) is false but (r) is true
The MTT assay determines cell viability based on the conversion of the tetrazolium salt into an insoluble formazan product by metabolically active cells, not DNA fragmentation. Therefore, Assertion (a) is false, while Reason (r) is correct.
Sourav
