A 5250 base-pair long plasmid with 10 negative supercoils would have a linking number of __________, considering 10.5 base pairs per turn for B DNA.

Sourav PanAugust 18, 2024

Answer

Answer: 490

Explanation:
The linking number (Lk) of DNA is given by:
$Lk = \frac{\text{Total base pairs}}{\text{Base pairs per turn}}$
For a 5250 bp plasmid:
$Lk_0 = \frac{5250}{10.5} = 500$
With 10 negative supercoils:
$Lk = Lk_0 - 10 = 500 - 10 = 490$

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