Phenol-Sulfuric Acid Method for Total Carbohydrates

Phenol-Sulfuric Acid Method for Total Carbohydrates

What is Phenol-Sulfuric Acid Method for Total Carbohydrates? Objective of Phenol-Sulfuric Acid Method Principle of Phenol-Sulfuric Acid Method The principle of the Phenol-Sulfuric Acid Method centers on the reaction of carbohydrates with strong acid and heat, leading to the production of detectable compounds. This method involves the breakdown of carbohydrates—such as simple sugars, oligosaccharides, or … Read more

Osazone Test – Principle, Procedure, Result, Uses

Osazone Test for Carbohydrates Principle, Procedure, Result

What is Osazone Test? The Osazone test is a biochemical method used to identify reducing sugars. This test is also known as the Phenylhydrazine test, referring to the reagent employed in the procedure. It distinguishes reducing sugars based on the time it takes for the osazone complex to form. Carbohydrates, or sugars, are defined chemically … Read more

GOD-POD Method For The Estimation Of Blood Glucose

GOD-POD Method For The Estimation Of Blood Glucose

Glucose, a key sugar molecule, plays a vital role in the human body as the primary carbohydrate found in the bloodstream. Its importance lies in its function as the main source of energy for various body tissues, notably the brain. When glucose is metabolized, it undergoes a sequence of chemical processes that result in the … Read more

Folin-Wu Method For Estimation of Blood Glucose

Folin-Wu Method For Estimation of Blood Glucose

What is Folin-Wu Method? Principle of Folin-Wu Method Requirements Procedure of Folin-Wu Method Step 1: Preparation of Protein-Free Filtrate Step 2: Testing Procedure This process results in a color change proportional to the glucose concentration in the blood sample. By comparing the absorbance of the test sample against the standard, the glucose level in the … Read more

Determination of Lactose In Milk by Lane-Eynon Method

Determination of Lactose In Milk by Lane-Eynon Method

What is Lane-Eynon Method? Principle of Lane-Eynon Method The Lane-Eynon method is a titrimetric procedure that quantifies reducing sugars in a solution through the utilization of Fehling’s solution as the primary reagent. This method operates on the fundamental chemistry of reduction-oxidation reactions, where Fehling’s solution, composed of copper sulfate and an alkaline solution of sodium … Read more

Determination of Calcium In Milk

Determination of Calcium In Milk

Milk is widely recognized as a significant source of calcium, an essential mineral that plays a crucial role in the development and maintenance of strong bones and teeth. This mineral is particularly important for infants and young children, whose rapidly growing bodies demand high levels of calcium to ensure proper bone formation and density. Calcium’s … Read more

Determination of Magnesium In Milk

Determination of Magnesium In Milk

Magnesium is an essential mineral found in many foods, including milk. It plays a vital role in numerous bodily functions, such as muscle and nerve function, blood sugar control, and bone health. The magnesium content in milk contributes to its nutritional value, making milk a beneficial part of a balanced diet. The amount of magnesium … Read more

Determination of Phosphorus In Milk

Determination of Phosphorus In Milk

Phosphorus is an essential mineral found in many foods, including milk. It plays a critical role in various bodily functions such as the formation of bones and teeth, energy production, and the synthesis of RNA and DNA, which are crucial for the growth and repair of cells. In milk, phosphorus is naturally present and contributes … Read more

Litmus Milk Test – Principle, Procedure, Result

Litmus Milk Test Principle, Procedure, Result

What is the Litmus Milk Test? Litmus Milk Medium is a liquid medium suggested for use in qualitative tests for determining the activity of bacteria on milk and maintaining lactic acid bacteria. Acid Reaction Alkaline Reaction Redox Reaction Principle of Litmus Milk Test Purpose of Litmus Milk Test Media Required This test utilises Litmus Milk … Read more

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