Molecular Basis of Diagnosis of Genetic Diseases – Cystic Fibrosis, Huntington’s, Sickle Cell Anaemia

Molecular Basis of Diagnosis of Genetic Diseases - Cystic Fibrosis, Huntington’s, Sickle Cell Anaemia

The early detection of diseases is crucial for effective treatment, and molecular diagnostics serves as a pivotal tool in this regard. This term encompasses a range of diagnostic techniques that assess an individual’s health at a molecular level. Specifically, molecular diagnostics focuses on the identification and quantification of genetic sequences—often referred to as biomarkers—found in … Read more

Biosensors – Principle, Types, Characteristics, Applications

Biosensors - Principle, Types, Characteristics, Applications

What are Biosensors? Definition of Biosensors A biosensor is a device that detects biological changes and converts them into an electrical signal, typically by combining a biological sensing element with a transducer for applications like medical diagnostics or environmental monitoring. Components of Biosensors Biosensors consist of several key components that work together to detect biological … Read more

High Throughput Sequencing (HTS) – Principle, Steps, Applications

High Throughput Sequencing (HTS)

What is High Throughput Sequencing (HTS)? Working Principle of High Throughput Sequencing (HTS) High-throughput sequencing (HTS) technologies High-throughput sequencing (HTS) technologies have revolutionized genomic research by enabling rapid and detailed analysis of genetic material. The principal HTS technologies can be categorized into next-generation sequencing (NGS) and third-generation sequencing. Each of these technologies has distinct methodologies, … Read more

Eukaryotic DNA Replication – Definition, Steps, Singnification

Eukaryotic DNA Replication

Have you ever wondered how your body creates new cells? Or how genetic information is passed down from one generation to the next? The answer lies in a fascinating process called DNA replication. In this article, we’ll dive deep into the world of eukaryotic DNA replication, exploring its intricacies, importance, and the cutting-edge research surrounding … Read more

Prokaryotic DNA Replication – Enzymes, Steps

Prokaryotic DNA Replication - Initiation, Elongation, Termination

What is Prokaryotic DNA Replication? Structure of Ori C OriC (Origin of Chromosome) is the specific locus in Escherichia coli where DNA replication is initiated. This critical region is located at approximately the 84.5 mpu position on the E. coli genome, situated opposite to the replication termination site. The structure of OriC is characterized by … Read more

Sanger Sequencing – Principle, Steps, Advantages, Uses

Sanger Sequencing - Principle, Steps, Advantages, Uses

What is Sanger Sequencing? Principle of Sanger Sequencing The principle of Sanger sequencing is rooted in the termination of DNA strand elongation by dideoxynucleotide triphosphates (ddNTPs). These ddNTPs are modified versions of normal DNA nucleotides that lack a 3’ hydroxyl group, which is essential for forming a phosphodiester bond. Without this bond, the DNA strand … Read more

Whole Genome Sequencing – Principle, Types, Steps, Applications

Whole Genome Sequencing - Principle, Types, Steps, Applications

What is Whole Genome Sequencing? Principle of Whole Genome Sequencing The principle of whole genome sequencing (WGS) revolves around the complete sequencing of an organism’s DNA, encompassing both coding and non-coding regions. This method provides a thorough understanding of the genome, detailing the genes, regulatory elements, and variations present. WGS begins with the extraction of … Read more

DNA Library – Types, Construction, Applications

DNA Library - Types, Construction, Applications

What is DNA Library (Genomic, cDNA)? Types of DNA Library There are two main types of DNA libraries: genomic libraries, and cDNA libraries. 1. Genomic Library 2. cDNA Library Genomic Library Construction Steps Genomic Library Construction Steps Creating a cDNA library begins with mRNA, which carries genetic instructions from DNA to ribosomes for protein synthesis. … Read more

Emulsion PCR – Principle, Procedure, Advantages, Limitations, Uses

Emulsion PCR - Principle, Procedure, Advantages, Limitations, Uses

What is Emulsion PCR? Principle of Emulsion PCR Emulsion PCR (polymerase chain reaction) is a sophisticated technique designed to amplify individual DNA templates in a high-throughput manner by partitioning the DNA into tiny water-in-oil droplets. Each droplet serves as an independent microreactor, allowing for the separate amplification of distinct DNA fragments. This method leverages the … Read more

TaqMan Probe – Definition, Principle, Applications

TaqMan - Definition, Principle, Applications

What is TaqMan Probe? Definition of TaqMan TaqMan probes are hydrolysis probes used in quantitative PCR (qPCR) to increase specificity by utilizing the 5´–3´ exonuclease activity of Taq polymerase to cleave a dual-labeled probe during hybridization, resulting in a fluorescence signal that allows for precise quantification of the target DNA. Principle of TaqMan TaqMan technology … Read more

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