Cresol Red Solution Preparation and Uses
Cresol red is used as a tracking dye for DNA/RNA in agarose gel electrophoresis and protein in polyacrylamide gel electrophoresis.
Editorial Team
Ethidium Bromide Solution Preparation and use
Ethidium bromide is used to visualize DNA in agarose gels and in many cesium chloride gradient protocols.
Chloroquine solution Preparation and Uses
It was originally used as an antimalarial compound. It kills the erythrocytic forms of malaria parasites at all stages of development but does not affect the sporozoites.
Bradford Stock Solution Preparation
This solution is mainly used to determine the concentration of proteins in solution.
5-Fluorocytosine (5-FC) Solution Preparation
5-fluorocytosine (5-FC) also known as Flucytosine. 5-Fluorocytosinewas first synthesized in 1957 as anti-tumor drug. It is an antifungal medication which is used with amphotericin B against Candida infections and cryptococcosis. This drug is mainly taken by mouth and by injection into a vein.
Hanging Drop Method (Motility test) Principle, and Procedure, Result, Uses
Hanging drop technique allows for the analysis of living microorganisms. This involves fixing the Microbial suspension into a drop of liquid over the slide of glass.
Lysine Iron Agar (LIA) Test Principle, Procedure, Result
What is the Lysine Iron Agar (LIA) Test? The solid medium Lysine Iron Agar (LIA) is indicated for use in qualitative techniques for the differentiation of microorganisms based on the generation of lysine decarboxylase and hydrogen sulphide. Purpose of Lysine Iron Agar (LIA) Test Principle of Lysine Iron Agar (LIA) Test Lysine Iron Agar Composition … Read more
Stokes Disc Diffusion Method – Principle, Procedure, Result.
Stokes disc diffusion method isn’t as well-standardized as Kirby-Bauer’s method and is utilized in labs especially where the exact amount of antimicrobial present in discs isn’t known because of the difficulty in getting discs and correctly storing them or when other requirements needed for the Kirby-Bauer method cannot be fulfilled.
Nagler Reaction – Lecithinase Test
Nagler’s Reaction or Lecithinase test is a test in biochemistry used to detect organisms that produce the phospholipases (lecithinases) e.g. Clostridium perfringens. Its alpha (a) toxin from C. perfringens exhibits the phospholipase enzyme activity, which aids in the distinction between C. perfringens and the other Clostridium species that generate the enzyme lecithinase (C.baratti, C.absonum, C.bifermantans, C.sordelli, and C.novyi) through neutralization of lecithin C activity with an antitoxin.
Widal Test – Principle, Procedure, Result, Advantages, Limitation
The Widal test is an agglutination test that determines any antibodies present in the patient’s serum that are generated against the causal agents of the enteric febrile illness (Salmonella Typhi, and Salmonella paratyphi B, A as well as C).