BNO Team 
3 min read 
Acridine Orange contains metachromatic properties, that’s why it is employed in fluorescence microscopy and flow cytometry study of cellular physiology and cell cycle state, including the fluorescent microscopic examination of microorganisms.
2 min read 
Toluidine blue is a basic thiazine metachromatic dye that stains nuclei blue, and can be used to differentiate different types of granules (e.g. within mast cells).
11 min read 
There are two methods which are commonly used to stain bacterial endospore such as Schaeffer Fulton and Klein methods.
6 min read 
Capsule staining is a differential staining method, where two different stains are used such as the primary stain and the counterstain.
4 min read 
Flagella can not be seen under a bright-field microscope by using ordinary stains. A simple and useful method has been used for visualization of flagella is known as wet mount technique.
5 min read 
In 1930, Leifson introduced a simple flagella stain, using this stain he observed that mutations leading to nonflagellated from flagellated bacteria were common, as well as mutation leading to loss of motility without loss of the flagellum itself.
2 min read 
Production of parasporal crystals is a unique ability (among Bacillus species) of Bacillus thuringiensis. This stain is a means of rapid identification of the species.
10 min read 
Sudan Black is a slightly basic dye and will combine with acidic groups in compound lipids, thus staining phospholipids also. An alternative stain to the Sudan Black B stain.
9 min read 
lactophenol cotton blue definition Lactophenol Blue Solution or Lactophenol cotton blue is a wet mounting medium and staining agent which is used for the preparation of microscopic slides of fungi for examination. Fungal elements are stained intensely blue. You might be thinking, why should we learn this technique or what is the purpose of this … Read more
10 min read 
Ziehl Neelsen stinging method is used to differentiate between the acid-fast and non-acid fast bacteria.