Collagen Hybridizing Peptide Staining
Collagen Hybridizing Peptide stain is a vital stain used for the detection of collagen degraded tissues, mainly used in Developmental Biology, Histology, and Histopathology.
BNO Team
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Iron-Hematoxylin Staining – Principle, Procedure, Result
Hematoxylin is extracted from a leguminous plant, known as Haematoxylon campechianum, thus it is a natural dye. It forms hematein by oxidation (Ripening).
Acridine Orange Staining
Acridine Orange contains metachromatic properties, that’s why it is employed in fluorescence microscopy and flow cytometry study of cellular physiology and cell cycle state, including the fluorescent microscopic examination of microorganisms.
Toluidine blue stain of Mast Cell
Toluidine blue is a basic thiazine metachromatic dye that stains nuclei blue, and can be used to differentiate different types of granules (e.g. within mast cells).
Endospore Staining – Principle, Procedure, Result
There are two methods which are commonly used to stain bacterial endospore such as Schaeffer Fulton and Klein methods.
Capsule Staining – Procedure, Principle, Result
Capsule staining is a differential staining method, where two different stains are used such as the primary stain and the counterstain.
Flagella Staining – Principle, Procedure, Result
Flagella can not be seen under a bright-field microscope by using ordinary stains. A simple and useful method has been used for visualization of flagella is known as wet mount technique.
Bacterial Flagella Staining by Leifson Flagella Stain Method
In 1930, Leifson introduced a simple flagella stain, using this stain he observed that mutations leading to nonflagellated from flagellated bacteria were common, as well as mutation leading to loss of motility without loss of the flagellum itself.
Parasporal Crystal Stain – Principle, Procedure, Result
Production of parasporal crystals is a unique ability (among Bacillus species) of Bacillus thuringiensis. This stain is a means of rapid identification of the species.
Sudan Black B Staining – Principle, Procedure, Applications
Sudan Black is a slightly basic dye and will combine with acidic groups in compound lipids, thus staining phospholipids also. An alternative stain to the Sudan Black B stain.