Join Our Whatsapp and Telegram Channel to Get Free eBooks Telegram | Whatsapp

Anesthetic (MS222) Solution Preparation

Anesthetic (MS222) Solution

  • Anesthetic (MS222) Solutionalso known as MS222 or TMS or Tricaine Methanesulfonate.
  • The FDA approved two products are Finquel (Argent Chemical Laboratories, Inc., 8702 152 Ave. NE, Redmond,  WA 98052, NADA 42-427) and Tricaine-S (Western Chemical 1269 Lattimore Road Ferndale, WA 98248, ANADA 200-226 ), and also recommended by IACUC to use it.
  • MS222 is the only anesthetic recommended for application on fish that could be accepted as human food and requires a 21 day withdrawal period prior to release or harvest and is only labeled for food fish in the families Ictaluridae, Salmonidae, Esocidae, and Percidae, use with other species should be restricted to the hatchery or laboratory
  • 100 mg/mL solutions of MS222 can be stored in amber jars at 4 °C or -20 °C for 6 mo and still used to effectively anesthetize zebrafish.

Use of Anesthetic (MS222) Solution

  • Anesthetic (MS222) solution used as an anesthetic and euthanasia agent in fish, amphibians and other cold-blooded animals. 
  • Anesthetic Solution (Buffered MS222) is commonly used to temporarily immobilize fish and amphibians.

Preparation of Anesthetic (MS222) Solution

To prepare 1L of Anesthetic (MS222) Solution, the following materials are required;

ComponentAmountConcentration
MS222 (3-aminobenzoic acid ethyl ester; Sigma-Aldrich A5040) (mw: 165.19 g/mol)1 g0.0061 M
sodium bicarbonate (mw: 84.01 g/mol)700 mg0.0083 M

Procedure

  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 1 g of MS222 (3-aminobenzoic acid ethyl ester; Sigma-Aldrich A5040) to the solution.
  3. Add 700 mg of sodium bicarbonate to the solution.
  4. Add distilled water until volume is 1 L.
  5. The resulting pH should be ~7.0. Store in a foil-covered container at 4°C for up to 1 mo. Warm to room temperature before use.

References

Related Posts

Leave a Comment

This site uses Akismet to reduce spam. Learn how your comment data is processed.

A new weapon in the battle against antibiotic resistance 16 Important Skills Needed For A Successful Career in Bioinformatics Top 5 High-Paying Biotech Jobs in India (No PhD Required) Top Emerging Trends in Bioinformatics Important Skills Needed For A Successful Career in Bioinformatics Research reveals plant pathogens repurpose phage elements for bacterial warfare Scientists show the key role of spleen and extracellular vesicles in cryptic malaria infections Scientists reveal molecular link between glucose sensing and pyroptosis cell death Scientists reconstruct ancient genomes of the two most deadly malaria parasites to identify origin and spread What are TaqMan probes?
A new weapon in the battle against antibiotic resistance 16 Important Skills Needed For A Successful Career in Bioinformatics Top 5 High-Paying Biotech Jobs in India (No PhD Required) Top Emerging Trends in Bioinformatics Important Skills Needed For A Successful Career in Bioinformatics Research reveals plant pathogens repurpose phage elements for bacterial warfare Scientists show the key role of spleen and extracellular vesicles in cryptic malaria infections Scientists reveal molecular link between glucose sensing and pyroptosis cell death Scientists reconstruct ancient genomes of the two most deadly malaria parasites to identify origin and spread What are TaqMan probes?

Adblocker detected! Please consider reading this notice.

We've detected that you are using AdBlock Plus or some other adblocking software which is preventing the page from fully loading.

We don't have any banner, Flash, animation, obnoxious sound, or popup ad. We do not implement these annoying types of ads!

We need money to operate the site, and almost all of it comes from our online advertising.

Please add biologynotesonline.com to your ad blocking whitelist or disable your adblocking software.

×