Simmons citrate agar test – Procedure, Principle, Result, Example.

Simmons citrate agar test

About Simmons citrate agar test Simmons citrate agar test helps in detection of citrate utilizing bacteria. In this test, a specific medium is used called Simmons’ citrate agar which helps to differentiate among various Gram-negative enteric bacilli. Simmons’ citrate agar is a type of selective and differential medium. Which is used for the study of … Read more

Blood Cross Matching – Principle, Procedure, Result.

Cross Match

Cross Match is a laboratory test, performed prior to a blood transfusion to determine whether donor blood is compatible (or incompatible) with recipient blood.

Polymerase Chain Reaction (PCR) – Definition, Steps, Principle, Application

Polymerase chain reaction Steps

Polymerase chain reaction or PCR is a laboratory technique that is used to make multiple copies (millions or billions!) of a specific region of DNA in vitro (in a test tube rather than an organism).

Blood Grouping Test – Principle, Procedure, Result

Blood Grouping Principle and Procedure

The concept of Blood grouping was first discovered by Karl Landsteiner in 1901, who was an Austrian-American immunologist and pathologist. He received Nobel Prize in Physiology or Medicine in 1930 for this discovery.

Eastern blot – Protocol, Principle, Application

Eastern blot Protocol

A Molecular Biology Technique is used for the identification of Post Translation Modification mostly carbohydrate Epitopes by transferring onto a membrane and analyzed using a probe.

Dot Blot – Protocol, Principle, Definition.

Dot Blot Protocol, Principle, Definition

Dot Blot is a simplified technique of western blotting, which is mainly used for the detection of proteins.

Restriction Digest – Protocol, Principle, result

Restriction Digest Principle, Procedure, result

The restriction enzymes cleave the DNA molecule at a specific site or specific nucleotide sequence, is known as recognition sequences or Restriction sites.

Reverse Blood Grouping – Principle, Procedure, Result

Reverse Blood Grouping

Reverse grouping is defined as using the reagent cells with known ABO antigens and testing the patient’s serum for ABO group antibodies.

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