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Nitrate Reduction Test – Procedure, Principle, Result

Nitrate Reduction Test Procedure, Principle, Result

What is Nitrate Reduction Test? Purpose of Nitrate Reduction Test Principle of Nitrate Reduction Test Reactions of Nitrate Reduction Test Use of Zinc powder Use of  A Durham tube Requirements Composition of Nitrate broth (medium) Ingredients Gms / Litre Peptic digest of animal tissue 5.000 Meat extract 3.000 Potassium nitrate 1.000 Sodium chloride 30.000 Final … Read more

Blood Cross Matching – Principle, Procedure, Result.

Cross Match

Cross Match is a laboratory test, performed prior to a blood transfusion to determine whether donor blood is compatible (or incompatible) with recipient blood.

Blood Grouping Test – Principle, Procedure, Result

Blood Grouping Principle and Procedure

The concept of Blood grouping was first discovered by Karl Landsteiner in 1901, who was an Austrian-American immunologist and pathologist. He received Nobel Prize in Physiology or Medicine in 1930 for this discovery.

Eastern blot – Protocol, Principle, Application

Eastern blot Protocol

A Molecular Biology Technique is used for the identification of Post Translation Modification mostly carbohydrate Epitopes by transferring onto a membrane and analyzed using a probe.

Dot Blot – Protocol, Principle, Definition.

Dot Blot Protocol, Principle, Definition

Dot Blot is a simplified technique of western blotting, which is mainly used for the detection of proteins.

Restriction Digest – Protocol, Principle, result

Restriction Digest Principle, Procedure, result

The restriction enzymes cleave the DNA molecule at a specific site or specific nucleotide sequence, is known as recognition sequences or Restriction sites.

Reverse Blood Grouping – Principle, Procedure, Result

Reverse Blood Grouping

Reverse grouping is defined as using the reagent cells with known ABO antigens and testing the patient’s serum for ABO group antibodies.

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