Bacteriology 14 Views 1 Answers
Avatar for Sourav Pan
Sourav PanDecember 7, 2024

What is the purpose of CaCl₂ in bacterial transformation?

What is the purpose of CaCl₂ in bacterial transformation?

Answer
Please login to save the post
1
Avatar for Sourav Pan
Sourav PanMay 15, 2025

Calcium chloride is used to make bacterial cells competent for DNA uptake by destabilizing the cell membrane. The calcium ions neutralize the negative charges on both the bacterial cell membrane and the DNA, reducing repulsion and facilitating binding. The treatment also makes the membrane more permeable, enabling DNA to enter the cells during the heat shock step.

0
0 likes

Related Questions

Quiz Leaderboard

1st
Meenakshi
Score: 100%
Questions: 20
2nd
Debabrata
Score: 100%
Questions: 20
3rd
Sourav
Score: 90%
Questions: 20
4th
Nischal
Score: 90%
Questions: 10
5th
Priyanka
Score: 90%
Questions: 10
6th
Martin
Score: 65%
Questions: 20
7th
Vesnce
Score: 45%
Questions: 20
8th
Meenakshi
Score: 45%
Questions: 20
9th
Sabhyata
Score: 40%
Questions: 10
10th
Hector
Score: 40%
Questions: 10
  • Click "Create Mock Test" to start a quiz.
  • Select categories and the number of questions, then click "Submit" to begin.
  • After completing the quiz, enter your name and email, then click "Submit" to view your score.
  • Your score is shown as a percentage (e.g., 5 correct out of 10 is 50%).
  • The leaderboard displays your name, score percentage, and total questions answered.
Click here to Create Mock Test
⚠️
  1. Click on your ad blocker icon in your browser's toolbar
  2. Select "Pause" or "Disable" for this website
  3. Refresh the page if it doesn't automatically reload