Bacteriology 6 Views 1 Answers

Sourav PanLv 9December 7, 2024
What factors affect bacterial transformation?
What factors affect bacterial transformation?
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Sourav PanLv 9May 15, 2025
Several factors influence bacterial transformation efficiency, including: 1) Bacterial species and strain, as some are naturally more competent; 2) DNA concentration and purity; 3) Method of competence induction (e.g., chemical vs. electroporation); 4) Temperature and timing of heat shock or electrical pulse; 5) Growth phase of bacterial cells, as cells in the early log phase are typically more receptive to transformation; and 6) Recovery conditions after transformation.
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