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Sourav PanDecember 7, 2024

What are the four steps in bacterial conjugation?

What are the four steps in bacterial conjugation?

Sourav Pan
Sourav PanDecember 7, 2024

Answered

The process of bacterial conjugation can be divided into four key steps: (1) Contact Formation: The donor cell produces a pilus that attaches to the recipient cell. This pilus acts as a physical conduit for genetic transfer. (2) Formation of the Mating Pair: The pilus retracts, bringing the donor and recipient cells into close proximity and forming a stable connection for DNA transfer. (3) DNA Transfer: A single strand of the donor’s plasmid or chromosomal DNA is transferred to the recipient cell while simultaneously being replicated in the donor to maintain its genome. (4) Recipient Cell Conversion: The transferred DNA is replicated in the recipient cell, completing the double-stranded DNA structure. If the transferred DNA includes genes for conjugation, the recipient can become a donor cell.

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