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To calibrate a pipette, a precise weighing procedure is usually followed using distilled water and a high-precision balance. First, clean the pipette thoroughly and attach a clean pipette tip. Set the pipette to a known volume (e.g., 1 mL) and draw liquid into the pipette. Then, dispense the liquid into a pre-weighed container or vessel and weigh it using a high-precision balance. Since 1 mL of distilled water weighs approximately 1 gram, compare the actual weight of the dispensed liquid to the expected weight. If the weight differs from the expected value, the pipette may need adjustment or recalibration. This process should be repeated for different volumes to ensure the pipette is accurate across its full range. Regular calibration is important for maintaining the accuracy of pipettes in the laboratory. Calibration should be performed according to the manufacturer’s instructions or by a qualified technician if needed.
BNO Team