Pyronin Y (PY) Solution Preparation and Application

By BNO Team Published on May 14, 2024 1 min readby BNO Team

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Pyronin Y (PY) Solution Application

Pyronin Y(G) (PY) is a dye used for DNA staining. PY has orange spectral emission that is easily distinguishable from FITC and 7-AAD.

Components Require

The following components are required to prepare 1L of Pyronin Y (PY) Solution.

Component	Amount	Concentration
Pyronin Y(G) (PY) (Polysciences, Inc.) (mw: 302.8 g/mol)	1 g	0.0033 M

Pyronin Y (PY) Solution Preparation

1. Prepare 800 mL of distilled water in a suitable container.
2. Add 1 g of Pyronin Y(G) (PY) (Polysciences, Inc.) to the solution.
3. Add distilled water until volume is 1 L.
4. Store at 4°C protected from light.

References

“Pyronin Y (PY) Solution Preparation.” AAT Bioquest, Inc, 02 Jun. 2021, https://www.aatbio.com/resources/buffer-preparations-and-recipes/pyronin-y-g-py-stock-solution-1-mg-ml.

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