What is Wheatley Trichrome Staining? In parasitology, intestinal protozoans are typically detected and identified from faeces samples using a specialised permanent technique called Wheatley Trichrome staining. Wheatley Trichrome Stain is suggested for use in qualitative methods for the detection, identification, and distinction of intestinal protozoa from background material. Typically, PVA-fixed or Schaudinn’s solution-preserved faecal specimens … Read more

Since the late 1950s and early 1960s, the positive staining approach has been employed to increase the contrast of biological materials (tissues and cell structures, viruses, etc.). The samples are treated in heavy metal salt solutions that react with cellular structures using this technique and negative staining. Uranyl acetate and lead citrate are the most … Read more

Trichrome stains, such as Masson’s Trichrome, derive its name from the three dyes utilised in the staining process. These three dyes are used to selectively stain muscle, collagen fibres, fibrin, and erythrocytes using acid-base chemistry. Initial placement of tissue slices in Bouin’s solution. As a mordant, Bouin’s solution links the dye to the specific tissue … Read more

In 1961, Darken reported the uptake of calcofluor white, a fluorescent brightener, by actively growing yeasts and higher fungi. Hageage and Harrington outlined the application of calcofluor white (CFW) to demonstrate hyphae and yeasts in clinical specimens. Monheit et al. utilised this dye for intraoperative identification of fungal infection on frozen sections of lung and … Read more

Grocott-Gomori’s Methenamine Silver Staining is a type of special staining technique used in microbiology and histology to visualize fungal organisms, specifically those that form characteristic spores known as arthroconidia. The staining technique was developed in the 1930s by Grocott and Gomori and is based on the use of silver and methenamine. The fungal cells are … Read more

What is silver staining? Silver staining is a powerful and versatile technique used for the detection and identification of proteins in gels. This technique is accomplished by binding silver to the chemical terminal or side chains of amino groups, such as carboxyl and sulfhydryl groups. For decades, silver staining has been used to separate proteins … Read more

What is Auramine- Rhodamine Staining? Principle of Auramine- Rhodamine Staining Auramine and Rhodamine are nonspecific fluorochrome dyes that are attracted to acid-fast microorganisms. In the case of Mycobacteria, the dyes can selectively bind to the mycolic acid present in the cell wall, allowing the stain to penetrate. This compound is resistant to acid-alcohol decolorizer solutions. … Read more