Malonate Test – Principle, Procedure, Results, Uses
The purpose of the malonate test is to see if the organism can get all of its energy from malonate.
Biochemical Test
Biochemical tests are laboratory procedures that use specific chemical reactions to identify and characterize microorganisms, such as bacteria. These tests are often used to identify the presence of specific enzymes or metabolic pathways in a microorganism, which can help to distinguish it from other microorganisms.
Some common biochemical tests used in bacteriology include:
Oxidase test: This test is used to detect the presence of the enzyme cytochrome oxidase, which is involved in the metabolism of oxygen.
Catalase test: This test is used to detect the presence of the enzyme catalase, which helps to break down hydrogen peroxide.
Indole test: This test is used to detect the presence of the enzyme tryptophanase, which breaks down the amino acid tryptophan.
Nitrate reduction test: This test is used to detect the ability of a microorganism to reduce nitrate to nitrite.
Gelatinase test: This test is used to detect the ability of a microorganism to produce the enzyme gelatinase, which breaks down gelatin.
These are just a few examples of the many different biochemical tests that are used in bacteriology. Biochemical tests can be useful for identifying and classifying bacteria, and for understanding their metabolism and ecological role.
Microdase Test – Principle, Procedure, Result, Uses
The oxidase enzyme is a key factor in distinguishing Staphylococcus from Micrococcus, and the Microdase Disk is a reagent-impregnated disc recommended for use in qualitative procedures.
API (Analytical Profile Index) 20E Test Result, Principle, Procedure
What is API (Analytical Profile Index) 20E Test? The API (Analytical Profile Index) 20E test is a standardized biochemical identification system used for the identification of Enterobacteriaceae and other non-fastidious Gram-negative rods. It is developed as a miniaturized system where a number of biochemical reactions are carried out simultaneously in a single plastic strip. It … Read more
Novobiocin Susceptibility Test – Principle, Procedure, Results
The novobiocin (antibiotic) differentiation disc can be used in the Novobiocin Susceptibility Test to help make a preliminary distinction between Staphylococcus saprophyticus and other coagulase-negative staphylococci (CoNS) in clinical (urinary) specimens.
OF (Oxidation-Fermentation)Test – Principle, Purpose, Procedure
The Oxidation–Fermentation (OF) test is a biochemical test that is used to differentiate bacteria on the basis of their ability to metabolize carbohydrates by oxidative or fermentative pathways. It is the process that helps in identifying whether the organism utilizes carbohydrate in the presence of oxygen or in the absence of oxygen. This test was … Read more
Pyruvate Broth Test – Principle, Purpose, Procedure, Result
Pyruvate Broth Test is a biochemical test that is used in microbiology for identification and differentiation of certain bacteria. It is mainly used for differentiating species of Enterococcus group. It is the process in which ability of organism to utilize sodium pyruvate as a sole source of energy is determined. The medium used in this … Read more
ONPG Test – Principle, Procedure, Result, Uses
The o-Nitrophenyl-β-D-Galactopyranoside (ONPG) test is a biochemical test used for the detection of the enzyme β-galactosidase in bacteria. It is mainly applied for differentiating late lactose fermenting organisms from non-lactose fermenters. It is based on the ability of the organism to hydrolyse a synthetic lactose analogue when the required enzyme is present. The test is … Read more
Salt Tolerance Test – Principle, Purpose, Procedure, Result
The ability to develop in the presence of varying concentrations of sodium chloride (NaCl) has been used to distinguish between various bacteria.
Sulfur Reduction Test – Principle, Purpose, Procedure, Result
The Sulfur Reduction Test is the biochemical procedure that is used to detect whether a microorganism is capable of reducing the sulfur-containing compounds to produce hydrogen sulfide (H₂S). It is the process which mainly helps in identifying members of Enterobacteriaceae where some bacteria like Salmonella, Proteus and Citrobacter are able to form the gas during … Read more
Growth at 42 Test – Principle, Procedure, Result, Uses
Growth at 42°C test is a physiological test used in microbiology for the identification and differentiation of certain non-fermentative Gram-negative bacteria. It is the process where the organism is tested for its ability to grow and survive at a higher temperature of 42°C. This test is mainly applied for the differentiation of Pseudomonas aeruginosa from … Read more