SOS Repair – SOS Response in Bacteria

SOS Repair - SOS Response in Bacteria

What is SOS Response or SOS Repair? Definition of SOS Response or SOS Repair The SOS response or SOS repair is a cellular mechanism activated in response to DNA damage, where the cell cycle is temporarily halted to facilitate DNA repair and mutagenesis. Central to this system is the RecA protein, which, when stimulated by … Read more

cDNA Synthesis – Principle, Protocol 

cDNA Synthesis - Principle, Steps

What is cDNA?  cDNA synthesis principle cDNA synthesis steps There are different steps for synthesis of cdna such as; cDNA synthesis protocol  1. Prepare sample 2. Remove genomic DNA 3. Select reverse transcriptase Common reverse transcriptases and their attributes.   AMV reverse transcriptase MMLV reverse transcriptase Engineered MMLV reverse transcriptase(e.g., Invitrogen SuperScript IV Reverse Transcriptase) RNase … Read more

Gene Cloning – Steps, Definition, Applications

Gene Cloning - Steps, Definition, Applications

What is Gene cloning? “DNA Cloning” is a molecular biology technique that is utilized to create exact copies, or clones, of one particular gene or DN.” History of Gene cloning Principle of Gene Cloning Requirements for Gene Cloning Restriction Enzyme Digestion and Ligation Enzyme Source Recognition Sequence Restriction Digest EcoRI Escherichia coli 5’GAATTC3’CTTAAG   5′—G↓AATTC—3’3′—CTTAA↑G—5′ … Read more

DNA polymerase IV – Definition, Structure, Mechanism, Functions

DNA polymerase IV - Definition, Structure, Mechanism, Functions

What is DNA polymerase IV? Definition of DNA polymerase IV DNA polymerase IV is a prokaryotic enzyme encoded by the dinB gene, involved in mutagenesis and DNA repair processes, especially during the SOS response to DNA damage. Unlike many polymerases, it lacks proofreading activity, making it error-prone, but it plays a crucial role in translesion … Read more

Deletion Mutation – Definition, Causes, Mechanism, Examples

Deletion Mutation - Definition, Causes, Mechanism, Examples

What is Deletion Mutation? Definition of Deletion Mutation A deletion mutation is a genetic anomaly in which a segment of a chromosome or DNA sequence is omitted during DNA replication, leading to the absence of specific nucleotides or entire chromosomal segments. This can result in altered gene function or expression. Causes of Deletion Mutation Deletion … Read more

Watson and Crick DNA Model

Watson and Crick DNA Model

In 1953, James Watson and Francis Crick made a groundbreaking contribution to the understanding of DNA structure. By combining physical and chemical data, they proposed a model for DNA as a double helix, a twisted molecule consisting of two complementary strands held together by hydrogen bonds. This model revolutionized our knowledge of genetics and unlocked … Read more

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