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Oxidase Test – Definition, Principle, Procedure, Result, Application

Oxidase Test Definition, Principle, Procedure, Result, Application

The oxidase test is a biochemical reaction that assays for the presence of cytochrome oxidase, an enzyme sometimes called indophenol oxidase. In the presence of an organism that contains the cytochrome oxidase enzyme, the reduced colorless reagent becomes an oxidized colored product .

Tollens’ Test Definition, Principle, Procedure, Result, Application.

Tollens’ Test Definition, Principle, Procedure, Result, Application.

It is a type of biochemical test which is used to distinguish reducing sugars from non-reducing sugars. This biochemical test is also known as the silver mirror test based on the end product of this test. This test was also used to differentiate between aldehydes and ketones through routine qualitative organic analysis.

Biuret Test For Protein Principle, Procedure, Result, Application.

Biuret Test For Protein Principle, Procedure, Result, Application.

Proteins are polymers of amino acids. They are complex organic compounds containing nitrogen, hydrogen, carbon, and oxygen. Proteins are abundant in our everyday food e.g. egg, soya bean, pulses, fish, milk etc.

CAMP Test – Principle, Procedure, Result

Christie, Atkins, and Munch-Peterson (CAMP) test

CAMP Test was first discovered by four researchers Christie, Atkins, Munch, and Peterson in 1944, that’s why this test is also known as Christie–Atkins–Munch-Peterson test.

Reverse CAMP test Principle, Procedure, Result

Reverse CAMP test

In this method, the Group B Streptococcus (CAMP test positive) is streaked in the center of sheep blood agar, and Clostridium perfringens is streaked perpendicular to it.

Gelatin Hydrolysis Test – Purpose, Principle, Procedure, Result.

Gelatin Hydrolysis Test Purpose, Principle, Procedure, Result.

The gelatin proteins derived from the animal connective tissue, collagen. Gelatin is produced when collagen is boiled in water. A gelatin hydrolysis test is used to detects the presence of gelatinases. Gelatinases are extracellularly secreted by some bacteria which hydrolyze or digest gelatin.

Simmons citrate agar test – Procedure, Principle, Result, Example.

Simmons citrate agar test

About Simmons citrate agar test Simmons citrate agar test helps in detection of citrate utilizing bacteria. In this test, a specific medium is used called Simmons’ citrate agar which helps to differentiate among various Gram-negative enteric bacilli. Simmons’ citrate agar is a type of selective and differential medium. Which is used for the study of … Read more

Total White Blood Cell (WBC) Count – Total Leucocyte Count (TLC)

Total White Blood Cell (WBC) Count - Total Leucocyte Count (TLC)

The White Blood Cells (WBCs) also known as Leucocytes are round nucleated cells in blood, that provide protection against foreign invaders. There are many types of White Blood cells found in blood, and identified as Granulocytes (Neutrophils, Eosinophils & Basophils) and Agranulocytes (Monocytes and Lymphocytes). The size of White Blood Cells can vary between 10 … Read more

Centrifuge Blood Sample – Purpose, Steps, Techniques

Centrifuge Blood Sample - Purpose, Steps, Techniques

What are the components of blood? Blood is a fluid tissue that circulates throughout the body and performs a number of important functions. It is made up of several components, including cells and plasma. Cells: Plasma: Plasma is the liquid portion of the blood that carries the cells and other substances throughout the body. It … Read more

Reticulocyte Count – Principle, Procedure, Calculations and Clinical Significances

Reticulocyte Count : Principle, Procedure, Calculations and Clinical Significances

What is Reticulocytes? Reticulocytes are immature red blood cells (erythrocytes) that have not yet completed the final stages of maturation. They are usually produced in the bone marrow and have a characteristic reticulated (network-like) appearance when stained with special dyes. Reticulocytes are important in the evaluation of anemia, as they provide information about the bone … Read more

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