Sucrose gradient centrifugation Definition Technique for the characterization and preparation of subcellular particles. A sucrose gradient is used to fill a centrifuge tube of the type swinging-bucket design. The bottom is the most dense, and the top the least dense. The solution is then layered with a suspension of the particles. Centrifugation separates particles in … Read more

Centrifugation of blood  The process of dividing whole blood into its components is known as blood separation. For research purposes, diagnostic and therapeutic reasons as well as research, whole blood may be separated. Scientists may employ several blood separation methods to achieve this. Blood separation machines that spin at high speeds exert a centripetal force, … Read more

Filtration is the most popular method to sterilize heat sensitive liquid and gases, without exposure to denaturing temperatures. Instead of killing microorganisms that cause contamination the process simply eliminates them. It is the process of choice to sterilize chemical solutions for antibiotics radioisotopes, toxic chemicals or vaccines, as well as carbohydrates, all of which are … Read more

Radiation is used to sterilize and decontaminate medical supplies (surgeon supplies, vaccines and drugs) as well as the food and beverage industries. There are two types of radiations available: ionizing or non-ionizing. Radiation sterilization isn’t widely used in the food industry because of concerns about radioactive contamination, toxic or carcinogenic products production, taste alteration, and … Read more

Direct Microscopic Count (DMC) Cell Counting Formula In Counting Chamber The number of bacterial cell per milliliter (ml) = Number of cells counted X dilution (if used) X 50,000* The factor of 50,000 is used in order to determine the cell count for 1 ml: 1 ml = 1000 mm3 = (50 times the chamber … Read more

Binomial nomenclature means a system that is used to provide a unique name to organisms, including plants, animals, microorganisms, and other living things. This naming system makes it easier to understand them better and also separates each species from others. Therefore a universally accepted naming system is essential. Binomial nomenclature definition Binomial nomenclature is a … Read more

The food supply upon which microorganisms thrive in laboratories is termed as a culture medium or culture media. Culture Medium Definition The nutrient preparation on or in which a culture (microorganism) is grown in the laboratory is known as culture medium or culture media. Classification of culture media/Types of Culture Medium Many special-purpose media are … Read more

Continuous Culture Definition Continuous Culture is an ‘open’-culture system for the cultivation of microorganisms or cells in which fresh sterilized medium is introduced at a steady flow rate and from which the culture fluid emerges at the same rate. Continuous Culture Advantages System applied for Continuous Culture There are two systems used for Continuous Culture … Read more

Synchronous culture Definition A synchronous or synchronized culture is a microbiological culture or a cell culture which contains the cells that are all in the same growth stage. Obtaining Synchronous culture  There are present different method for obtaining a Synchronous culture such as; Application of Synchronous culture References

What is Diauxic growth? When a bacterium is incubated in a medium containing two sugars one monosaccharide and another di or polysaccharide (like glucose and lactose). The bacteria first utilize glucose and complete their lag and log phase. After completion of glucose utilization, they used lactose and produce and another lag phase due to the … Read more