Mannitol Salt Agar (MSA) – Composition, Principle, Preparation, Results, Uses

Mannitol Salt Agar (MSA)

Mannitol Salt Agar (MSA) is utilized as a differential and selective medium to isolate and detect Staphylococcus aureus in clinical and non-clinical samples. It is recommended for identification and enumeration of coagulase positive Staphylococci that are present in milk, food as well as other specimens. MSA also promotes an increase in the number of particular group of specific bacteria, while limiting the growth of other bacteria.

New York City Agar – Composition, Preparation, Result, Uses

New York City Agar Composition, Preparation, Result, Uses

NYC Agar Base medium was initially created by Fauer, Weisburd and Wilson at the New York City Department of Health to isolate the pathogenic Neisseria strains from human samples. NYC medium, designed primarily to isolate pathogenic Neisseria is also able to support the growth of mycoplasmas with large colonies or mycoplasmas T (Ureaplasma). It is a translucent medium, highly selective, allowing direct microscopic observations and presumed identification of mycoplasmas and Neisseria gonorrhoeae.

Martin Lewis Agar – Composition, Preparation, Principle, Uses

Martin Lewis Agar Composition, Preparation, Principle, Uses

What is Thayer Martin Medium? Composition of Martin Lewis Agar Ingredients Gms/L Casein Peptone 7.5 Meat Peptone 7.5 Corn Starch 1.0 Potassium Phosphate, dibasic 4.0 Potassium Phosphate, monobasic 1.0 Sodium Chloride 5.0 Agar 10.0 Hemoglobin 10.0 Dextrose 1.5 Enrichment Bio-X 10 ml Antibiotics V.C.A.T 10 ml pH 7.2 +/- 0.2 at 25º C Enrichment Bio-X … Read more

Peptone Water – Principle, Composition, Preparation, Uses

Peptone Water Composition, Preparation, Uses, Principle

Peptone Water is a type of broth medium. This media is mainly used to grow the organism and is also used as a base for determining carbohydrate fermentation patterns of non-fastidious organisms. It is also used to detect indole production by the organism.

Alkaline Peptone Water – Preparation, Composition, Principle, Application.

Alkaline Peptone Water Preparation, Composition, Principle, Application.

Clinical materials containing small numbers of Vibrio should be inoculated into an enrichment medium prior to plating onto a selective medium, such as TCBS Agar (M189). Alkaline Peptone Water is a suitable enrichment broth for this purpose. 

Blood Agar – Preparation, Composition, Application, and limitation

Blood Agar Definition, Preparation, Composition, Application, and limitation.

production of blood agar after the addition of liquid blood.

Sheep blood agar – Composition, Principle, Procedure, Uses.

Sheep blood agar Composition, Principle, Procedure, Uses.

Sheep blood agar The principle of sheep blood agar Haemolysins are exotoxins created by bacteria that kill Red blood cell clots. The haemolytic process can be observed on blood Agar Plates. In blood agar plates, colonies of haemolytic bacterium may be covered by a the clear, uncoloured zone, in which the Red blood cells are … Read more

Mueller Hinton Agar (MHA) Composition, Principle, Preparation

Mueller Hinton Agar (MHA) Composition, Principle, Preparation

What is Mueller Hinton Agar (MHA)? Why MHA is used for antibiotic susceptibility testing (AST)? Mueller Hinton Agar (MHA) is commonly used for antibiotic susceptibility testing due to several key reasons: Mueller Hinton Agar (MHA) Principle The principle of Mueller Hinton Agar (MHA) revolves around its composition and the controlled levels of various components to … Read more

Salmonella Shigella Agar (SS Agar) – Principle, Composition, Application

Salmonella Shigella (SS) Agar Principle, Composition, Application

Salmonella Shigella (SS) Agar can be moderately specific and a differing medium used for the cultivation, isolation as well as differentiation Salmonella spp. and a few varieties that belong to Shigella spp.

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