Lactobacilli MRS Broth and Agar – Composition, Preparation, Principle

Lactobacilli MRS Broth and Agar - Composition, Preparation, Principle

MRS Broth was developed by deMan, and co. in order to facilitate the most abundant growth of lactobacilli derived from feces, oral and other samples. Lactobacilli MRS Broth is a better media for lactobacilli. It allows for healthy growth and is especially suitable for several very discerning strains that grow poorly in other media.

Cystine Tryptic Agar – Composition, Preparation, Principle, Uses

Cystine Tryptic Agar - Composition, Preparation, Principle, Uses

Cystine tryptic agar (CTA) which is also referred to as cystine trypticase agar was created by Vera as an semi-solid growth medium to identify and maintain the health of microorganisms for long time period of time when stored at suitable temperatures. It’s used for determination of the motility of bacteria and, when combined with carbohydrate, is involved in the fermentation process and the differentiation of microorganisms that are fastidious, such as Neisseria and streptococci, as well as pneumococci and non-spore-forming anaerobes.

Sabouraud Dextrose Agar (SDA) – Composition, Preparation, Principle, Uses, Results

Sabouraud Dextrose Agar (SDA) - Composition, Preparation, Principle, Uses, Results

Raymond Sabouraud created Sabouraud Dextrose Aga or SDA in 1892. Sabouraud Dextrose Aggar is useful in the cultivation of fungi (yeasts and moulds), especially for skin infections.

Common Fungal Culture Media and Their Uses

Common Fungal Culture Media and Their Uses

To ensure that clinical specimens are properly cultured, two types of media are required. The non-selective medium (e.g. Brain Heart Infusion Agar) should be used. Other media should be specific and tailored to isolate pathogenic fungi.

Phenylethyl Alcohol Agar – Composition, Preparation, Uses, Limitation

Phenylethyl Alcohol Agar - Composition, Preparation, Uses, Limitation

A selective medium called phenylethyl alcohol (PEA), is used to cultivate Gram positive organisms, especially cocci, in a sample containing pathogens. Phenylethyl alcohol is the active ingredient that inhibits or significantly reduces growth of Gram-negative organisms.

Anaerobic Culture Media – Examples, Definition

Anaerobic Culture Media Examples, Definition

Anaerobic Culture Media used in anaerobic bacteriology may be prepared in a fresh manner or bought from commercial vendors.
Media for anaerobic fermentation that is created in the laboratory should be used within two weeks after the time of preparation, as prolonged storage can degrade the quality of the medium due to peroxide accumulation and dehydration.

Lowenstein Jensen (LJ) Media – Composition, Preparation, Uses

Lowenstein Jensen (LJ) Media Composition, Preparation, Uses

Lowenstein Jensen Medium (LJ Medium) is a highly selective medium.
Solid media that are used to isolate as well as cultivation of Mycobacteria can be egg-based or Agar-based.

Potato Dextrose Agar (PDA) – Principle, Composition, Preparation, Uses

Potato Dextrose Agar (PDA) Principle, Composition, Preparation, Uses

Potato Dextrose Agar (PDA) is commonly abbreviated as PDA.
Potato Dextrose Agar has been suggested by APHA and F.D.A.for the count of moulds and yeasts during the testing of food items and dairy products.

Chocolate Agar Media – Principle, Composition, Preparation, Result

Chocolate Agar Media Principle, Composition, Preparation, Result

Chocolate Agar (CAP or CHOC) is a nonselective enhanced medium that is used to identify and isolate of pathogens that are fastidious.

Xylose Lysine Deoxycholate (XLD) Agar – Composition, Preparation, Principle, Uses

Xylose Lysine Deoxycholate (XLD) Agar Composition, Preparation, Principle, Uses

Xylose Lysine Deoxycholate agar (XLD) is a selective medium that allows for the isolation and growth of Salmonella and Shigella species using clinical samples or food. Taylor developed XLD Agar to aid in the differentiation, isolation and identification of enteric disease agents and support the growth more specialized enteric organisms. XLD Agar is a well-proven medium that allows the growth of Shigella species. It is also an excellent medium to isolate Salmonella species. It has a pH value of 7.4, giving it a bright pink to red color due to the indicator phenol. 

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