Biochemical tests are laboratory procedures that use specific chemical reactions to identify and characterize microorganisms, such as bacteria. These tests are often used to identify the presence of specific enzymes or metabolic pathways in a microorganism, which can help to distinguish it from other microorganisms.
Some common biochemical tests used in bacteriology include:
Oxidase test: This test is used to detect the presence of the enzyme cytochrome oxidase, which is involved in the metabolism of oxygen.
Catalase test: This test is used to detect the presence of the enzyme catalase, which helps to break down hydrogen peroxide.
Indole test: This test is used to detect the presence of the enzyme tryptophanase, which breaks down the amino acid tryptophan.
Nitrate reduction test: This test is used to detect the ability of a microorganism to reduce nitrate to nitrite.
Gelatinase test: This test is used to detect the ability of a microorganism to produce the enzyme gelatinase, which breaks down gelatin.
These are just a few examples of the many different biochemical tests that are used in bacteriology. Biochemical tests can be useful for identifying and classifying bacteria, and for understanding their metabolism and ecological role.
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What is a Glucose Tolerance Test? A glucose tolerance test is a test that is used to diagnose diabetes or prediabetes. It involves measuring the body’s ability to process glucose, a type of sugar that is the main source of energy for the body. During the test, a person will drink a solution containing a … Read more
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Human pathogenic bacteria transmitted via the fecal-oral route, i.e., primarily intestinal pathogens, constitute one component of drinkable water quality analysis. Screening water for faecal contamination by testing for the presence of an indicator microbe is more practicable than doing thorough routine examinations for the presence of every type of disease. If you want to know … Read more
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The bulk of plasma proteins are composed of big particles, making it difficult for them to pass through the glomeruli of the kidney. However, kidney tubules filter out and reabsorb smaller protein particles back into the blood. There are traces of proteins such as Tamm-Horsfall protein, hormones, mucopolysaccharides, albumin, enzymes, and immunoglobulins in normal urine. … Read more
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The majority of plasma proteins are too big to pass through the kidney’s glomeruli. The limited quantity of protein that does filter through is often reabsorbed by the renal tubules back into the blood. Normal urine contains only trace levels of protein (less than 150 mg per 24 hours). Tamm-horsfall protein (up to 40%), albumin … Read more
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What is Pauly’s Test? Purposes of Pauly’s Test Principle of Pauly’s Test The presence of tyrosine and histidine can be determined with this test with high sensitivity. Sulphanilic acid is dissolved in hydrochloric acid and used as the reagent in this test. When being exposed to sodium nitrite and hydrochloric acid, sulphanilic acid forms a … Read more
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Many bacteria produce a group of enzymes known as beta-lactamases that are mediated through genes in plasmids or the chromosomes. Beta-lactamase can be a constant process or it may be triggered through contact with antimicrobials. Beta-lactamases hydrolyze (and consequently inhibit) the beta-lactam ring of many Cephalosporins as well as penicillins. Beta-Lactamase Test is a rapid test to determine the presence of beta-lactamase , an enzyme that is produced by the strains from Staphylococcus aureus Neisseria gonorrhoeae, Branhamella catarrhalis as well as Haemophilus influenzae.
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What is Urease Test? Purpose of Urease Test Principle of Urease Test (NH2)2CO + H2O → CO2 + 2NH3 Media Used for Urease Test Two types of media are typically employed to detect urease activity. Christensen’s urea agar is employed to identify urease activity in numerous bacteria. Stuart’s urea broth is largely used to distinguish … Read more
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Test Name Esculin Hydrolysis test Purpose This test is used to try to figure out what kind of Enterobacteriaceae it is and how it differs from other kinds.To see if the microbe can use esculin as a source of carbon by breaking it down with water. Media Required Esculin Hydrolysis Agar Result Positive: Blackened medium, … Read more