Biochemical tests are laboratory procedures that use specific chemical reactions to identify and characterize microorganisms, such as bacteria. These tests are often used to identify the presence of specific enzymes or metabolic pathways in a microorganism, which can help to distinguish it from other microorganisms.
Some common biochemical tests used in bacteriology include:
Oxidase test: This test is used to detect the presence of the enzyme cytochrome oxidase, which is involved in the metabolism of oxygen.
Catalase test: This test is used to detect the presence of the enzyme catalase, which helps to break down hydrogen peroxide.
Indole test: This test is used to detect the presence of the enzyme tryptophanase, which breaks down the amino acid tryptophan.
Nitrate reduction test: This test is used to detect the ability of a microorganism to reduce nitrate to nitrite.
Gelatinase test: This test is used to detect the ability of a microorganism to produce the enzyme gelatinase, which breaks down gelatin.
These are just a few examples of the many different biochemical tests that are used in bacteriology. Biochemical tests can be useful for identifying and classifying bacteria, and for understanding their metabolism and ecological role.
The majority of plasma proteins are too big to pass through the kidney’s glomeruli. The limited quantity of protein that does filter through is often reabsorbed by the renal tubules back into the blood. Normal urine contains only trace levels of protein (less than 150 mg per 24 hours). Tamm-horsfall protein (up to 40%), albumin … Read more
What is Pauly’s Test? Purposes of Pauly’s Test Principle of Pauly’s Test The presence of tyrosine and histidine can be determined with this test with high sensitivity. Sulphanilic acid is dissolved in hydrochloric acid and used as the reagent in this test. When being exposed to sodium nitrite and hydrochloric acid, sulphanilic acid forms a … Read more
Principle of PYR Test Purpose of PYR Test Procedure of PYR Test The PYR examination is accessible in many versions. The original PYR test took 16 to 20 hours, but a 4-hour broth assay is now available, as reported in this post. Other forms include quick (10-15 minute) assays in which organisms to be evaluated … Read more
What is Urease Test? Purpose of Urease Test Principle of Urease Test (NH2)2CO + H2O → CO2 + 2NH3 Media Used for Urease Test Two types of media are typically employed to detect urease activity. Christensen’s urea agar is employed to identify urease activity in numerous bacteria. Stuart’s urea broth is largely used to distinguish … Read more
Principle of Nitrite Reduction Test Nitrites combine with an acidic solution of sulfanilic acid and alpha-naphthylamine to produce a red azo dye. In each test reaction, the appearance of the red dye shows the presence of NO2- in the test tube, either as an unreduced main substrate, a product of the test organism’s reduction of … Read more
Cetrimide Selective Agar is a solid medium that is recommended for use in qualitative procedures for selective isolation and presumed identification of Pseudomonas aeruginosa and other nonfermenting, gram-negative bacilli.
Overview of Butyrate Test Test Name Butyrate Disk Test Detection Enzyme butyrate esterase Uses This is a quick way to find out if Moraxella catarrhalis has the enzyme butyrate esterase. Result A blue colour development Require Bromochloro-indolyl butyrate and 4-methylumbelliferyl impregnated discs Test Organism Moraxella catarrhalis ATCC 25240: butyrate positive Butyrate Disk Test Purpose Principle … Read more
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