Germplasm conservation Methods

Methods of Germplasm Storage and Conservation

What is Germplasm conservation? History of Germplasm Conservation Germplasm conservation has evolved through significant scientific and international efforts to safeguard the genetic diversity of crop plants. Below is a detailed historical account highlighting key milestones: Methods of Germplasm Storage and Conservation There are two methods for Germplasm Storage and Conservation A. Ex Situ Conservation Ex … Read more

Micropropagation – Definition, Application, Advantages, Disadvantages

Micropropagation - Definition, Application, Advantages, Disadvantages

What is Micropropagation? Micropropagation Definition Micropropagation is a method of rapidly multiplying plants by growing plant tissues, typically from the apical meristem, in sterile, controlled conditions to produce genetically identical clones. It is used for large-scale plant production, crop improvement, and maintaining genetic uniformity. Stages of Micropropagation – Steps of Micropropagation Micropropagation is a technique used … Read more

Applications of Plant Biotechnology

Applications of Plant Biotechnology

Genetically engineered crops Genetically engineered crops have emerged as a transformative component of modern agriculture, driven by advances in plant biotechnology. This innovative field enables the transfer of specific genes from various organisms into plants, leading to the development of crops with enhanced traits. The process of genetic engineering offers solutions to many agricultural challenges, … Read more

Transgenics in Crop Improvement – Insect, Viral, Disease, Abiotic Stresses resistance

Transgenics in Crop Improvement

Resistance of transgenic crops to biotic stresses Resistance to biotic stresses has been discussed under the following headings. Insect resistance of transgenics crops The development of transgenic crops with insect resistance represents a significant advancement in agricultural biotechnology. By incorporating specific insect control genes, particularly those derived from Bacillus thuringiensis (Bt), researchers have successfully engineered … Read more

BT Cotton – Definition, Features, Development, Advantages, Limitations

BT Cotton - Definition, Features, Development, Advantages, Limitations

What is BT Cotton? What is BT? Bt, short for Bacillus thuringiensis, is a widely recognized soil bacterium known for its insecticidal properties. This gram-positive and spore-forming bacterium produces unique parasporal crystals during its stationary growth phase, which play a crucial role in pest management. Features of Bt Cotton The following points highlight the key … Read more

Bt Crops – Definition, Types, Advantages, Limitations

The mechanism of Bt toxicity

What are Bt Crops? Definition of Bt Crops Bt crops, or Bacillus thuringiensis crops, are genetically modified plants that incorporate genes from the bacterium Bacillus thuringiensis. These crops produce specific proteins (Cry proteins) that are toxic to certain insect pests, providing built-in protection against infestations. Common examples include cotton, corn, and brinjal. Bt crops reduce … Read more

Applications of Biotechnology

Applications of Biotechnology

What is Biotechnology? Biotechnology is a broad field that involves the use of living organisms, cells, and biological systems to develop products and technologies that improve human life and the environment. It integrates knowledge from various scientific disciplines, including biology, chemistry, genetics, and engineering, to manipulate biological materials for specific applications. Here are some key … Read more

Bombardment (Biolistics) Method for Plant Cells – Principle, Requirement, Protocol

Bombardment (Biolistics) Method for Plant Cells - Principle, Requirement, Protocol

What is Bombardment (Biolistics) Method? Principle of Bombardment (Biolistics) Method Materials Required for Bombardment (Biolistics) Method Protocol for Bombardment (Biolistics) Method The bombardment method, also known as biolistics, is a sophisticated technique employed for introducing foreign DNA into plant cells. This protocol outlines the essential steps for preparing plant cells and DNA-coated microcarriers, executing the … Read more

Electrofusion Technique for Plants – Requirement, Protocol, Advantages, Uses

Electrofusion between a celery cell suspension protoplast and a lovage meso- phyll protoplast.

What is the Electrofusion of Plant Cells? Materials Required for Electrofusion Electrofusion of plant cells involves a variety of materials essential for the successful execution of the technique. The following is a comprehensive list of the materials required, each with a detailed explanation of its function in the electrofusion process: Electrofusion Protocol The following detailed … Read more

Agrobacterium-Mediated Gene Transfer in Plants

Agrobacterium-Mediated Gene Transfer in Plants

What is Agrobacterium-Mediated Gene Transfer? Agrobacterium-mediated Gene Transfer Principle Agrobacterium-mediated gene transfer is a sophisticated process that enables the genetic modification of plants through the natural infection mechanisms of the bacterium Agrobacterium tumefaciens. The underlying principle of this transformation hinges on the transfer of a segment of DNA, referred to as T-DNA, from the bacterium … Read more

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