Ion Exchange Chromatography – Principle, Protocol, Applications, Examples

Ion Exchange Chromatography – Definition, Principle, Protocol, Applications, Examples

What is Ion Exchange Chromatography? Definition of Ion Exchange Chromatography Ion exchange chromatography is a separation technique that separates ions and polar molecules based on their affinity to ion exchangers. It involves the reversible exchange of ions between the target ions in the sample solution and ions attached to an ion exchanger. Working Principle of … Read more

Ion exchange mechanism in Ion Exchange Chromatography

Ion exchange mechanism in Ion Exchange Chromatography

Ion exchange chromatography (IEC) is a chromatographic technique that allows for the separation of ions and polar molecules based on their charge. It utilizes a stationary phase composed of an insoluble matrix with covalently bonded charged groups. These charged groups can attract and bind ions of the opposite charge present in the mobile phase, which … Read more

Ocular Micrometer – Definition, Principle, Parts, Applications

Ocular Micrometer - Definition, Principle, Parts, Applications

What is Ocular Micrometer? Working Principle of Ocular Micrometer The principle of an ocular micrometer revolves around using a calibrated scale to accurately measure the size of objects viewed through a microscope. This scale, typically divided into 100 divisions, each measuring 0.01 millimeters (µm), is an essential component placed within the eyepiece of the microscope. … Read more

Toolmakers Microscope – Principle, Procedure, Parts, Applications

Toolmakers Microscope - Principle, Procedure, Parts, Applications

What is Toolmakers Microscope? Principle of Toolmakers Microscope The working principle of the toolmakers microscope relies on the principles of optics, similar to a conventional light microscope. The microscope is equipped with a light source that directs light upward, focusing it onto the object being observed. When the light falls on the object, it casts … Read more

Gradient PCR – Definition, Principle, Process, Functions

Gradient PCR - Definition, Principle, Process, Functions

What is Gradient PCR? Definition of Gradient PCR Gradient PCR is a specialized molecular technique that allows for the simultaneous testing of different annealing temperatures in a single PCR run, optimizing the conditions for precise DNA amplification. Principle of Gradient PCR The principle of Gradient PCR revolves around the capability to simultaneously evaluate a range … Read more

How to Calibrate ph Meter?

How to Calibrate ph Meter?

What is ph Meter? Why Calibrate A pH Meter? Calibration of a pH meter is a critical procedure to ensure the accuracy and reliability of the measurements it provides. Here are the primary reasons why calibration is essential: In summary, calibrating a pH meter is essential for maintaining its accuracy, ensuring the reliability of results, … Read more

Spectrophotometer Cuvette – Features, Types, Materials, Precautions, Advantages

Spectrophotometer Cuvette - Features, Types, Materials, Precautions, Advantages

What is Spectrophotometer Cuvette? A spectrophotometer cuvette is a small, usually rectangular container used to hold liquid samples in a spectrophotometer. Spectrophotometers are instruments that measure the amount of light absorbed by a sample as a function of wavelength. The cuvette is an essential component of these instruments because it holds the sample in place … Read more

Atomic Absorption Spectrophotometer

Atomic Absorption Spectrophotometer

What is Atomic Absorption Spectrophotometer? Definition of Atomic Absorption Spectrophotometer The Atomic Absorption Spectrophotometer (AAS) is a scientific instrument used to quantitatively determine the presence and concentration of specific chemical elements in a sample by measuring the absorbed wavelengths of light by free metallic ions in their gaseous state. Principle of Atomic Absorption Spectrophotometer The … Read more

Color Spectrophotometer – Principle, Parts, Procedure, Applications

Color Spectrophotometer - Principle, Parts, Procedure, Applications

What is Color Spectrophotometer? A color spectrophotometer is a scientific instrument designed to measure and analyze the color properties of various samples. By shining light onto a sample and measuring the intensity of the reflected light across different wavelengths, it provides a detailed profile of the sample’s color. This tool is essential in industries like … Read more

Bright Field Microscope – Definition, Parts, Working Principle, Application

Bright Field Microscope - Definition, Parts, Working Principle, Application

What is Bright Field Microscope? Bright Field Microscope Definition The Brightfield Microscope, often termed the Compound Light Microscope, is an optical instrument that utilizes light rays to produce a dark image against a bright background, primarily used in biological studies to observe stained specimens. Principle of Brightfield Microscope The Brightfield Microscope operates on a foundational … Read more

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