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BOD Incubator – Principle, Parts, Procedure, Application

BOD Incubator - Definition, Principle, Components, Procedure, Application

A BOD incubator is a laboratory instrument that is used for maintaining a controlled temperature environment required for the estimation of Biochemical Oxygen Demand of water samples. It is designed to operate both at heating and cooling conditions which is different from ordinary incubators. It is mainly adjusted at a fixed temperature of 20°C which … Read more

Lipase Test – Principle, Procedure, Results, Uses

Lipase Test Principle, Procedure, results

A lipase test is a laboratory diagnostic test which is done to measure the level of lipase enzyme in the blood. Lipase is a digestive enzyme which is mainly produced by pancreas and it helps in digestion of fats. It is the process in which dietary triglycerides are broken down into fatty acids and glycerol … Read more

Malonate Test – Principle, Procedure, Results, Uses

Malonate Test Principle, Procedure, Results

The purpose of the malonate test is to see if the organism can get all of its energy from malonate.

Microdase Test – Principle, Procedure, Result, Uses

Microdase Test Principle, Procedure, Result

The oxidase enzyme is a key factor in distinguishing Staphylococcus from Micrococcus, and the Microdase Disk is a reagent-impregnated disc recommended for use in qualitative procedures.

API (Analytical Profile Index) 20E Test Result, Principle, Procedure

API (Analytical Profile Index) 20E Test Result, Principle, Procedure

What is API (Analytical Profile Index) 20E Test? The API (Analytical Profile Index) 20E test is a standardized biochemical identification system used for the identification of Enterobacteriaceae and other non-fastidious Gram-negative rods. It is developed as a miniaturized system where a number of biochemical reactions are carried out simultaneously in a single plastic strip. It … Read more

Novobiocin Susceptibility Test – Principle, Procedure, Results

Novobiocin Susceptibility Test Principle, Procedure, Results

The novobiocin (antibiotic) differentiation disc can be used in the Novobiocin Susceptibility Test to help make a preliminary distinction between Staphylococcus saprophyticus and other coagulase-negative staphylococci (CoNS) in clinical (urinary) specimens.

Pyruvate Broth Test – Principle, Purpose, Procedure, Result

Pyruvate Broth Test Principle, Purpose, Procedure, Result

Pyruvate Broth Test is a biochemical test that is used in microbiology for identification and differentiation of certain bacteria. It is mainly used for differentiating species of Enterococcus group. It is the process in which ability of organism to utilize sodium pyruvate as a sole source of energy is determined. The medium used in this … Read more

ONPG Test – Principle, Procedure, Result, Uses

o-Nitrophenyl-b-D-Galactopyranoside (ONPG) Test Principle, Procedure

The o-Nitrophenyl-β-D-Galactopyranoside (ONPG) test is a biochemical test used for the detection of the enzyme β-galactosidase in bacteria. It is mainly applied for differentiating late lactose fermenting organisms from non-lactose fermenters. It is based on the ability of the organism to hydrolyse a synthetic lactose analogue when the required enzyme is present. The test is … Read more

Salt Tolerance Test – Principle, Purpose, Procedure, Result

Salt Tolerance Test Principle, Purpose, Procedure, Result

The ability to develop in the presence of varying concentrations of sodium chloride (NaCl) has been used to distinguish between various bacteria.

Sulfur Reduction Test – Principle, Purpose, Procedure, Result

Sulfur Reduction Test - Principle, Purpose, Procedure, Result

The Sulfur Reduction Test is the biochemical procedure that is used to detect whether a microorganism is capable of reducing the sulfur-containing compounds to produce hydrogen sulfide (H₂S). It is the process which mainly helps in identifying members of Enterobacteriaceae where some bacteria like Salmonella, Proteus and Citrobacter are able to form the gas during … Read more

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