Casein Hydrolysis Test – Principle, Procedure, Result, Uses

Casein Hydrolysis Test Principle, Procedure, Result

The Casein Hydrolysis Test is a biochemical test that is used to determine the ability of microorganism to produce and secrete extracellular proteolytic enzyme known as caseinase. It is the process in which casein protein of milk is broken down into smaller soluble products. Casein is the main protein present in milk and it is … Read more

Gram-Sure Test -Principle, Procedure, Result, uses

Gram-Sure (L-Alanine-7-amido-4-methylcourmarin) Test 

Gram-Sure Test is a rapid qualitative diagnostic test used for the differentiation of aerobic Gram-negative and Gram-positive bacteria. It is mainly applied for bacteria that are rods or coccobacilli in shape. This test works by detecting the presence of a specific enzyme known as L-alanine aminopeptidase. This enzyme is present almost exclusively in Gram-negative bacterial … Read more

Esculin Hydrolysis test – Principle, Procedure, Result, Uses

Esculin Hydrolysis test Principle, Procedure, Result

The Esculin hydrolysis test is a biochemical test which is used for the identification and differentiation of certain bacteria, mainly Enterococcus species and Group D streptococci from other streptococci. It is also used for identifying some other organisms such as Listeria species and members of Bacteroides fragilis group. It is based on the ability of … Read more

Bile Solubility Test – Principle, Procedure, Result, Uses

Bile Solubility Test - Principle, Procedure, Result

It is a biochemical test used in microbiology for the identification of Streptococcus pneumoniae from other alpha hemolytic streptococci. It is mainly based on the ability of pneumococcal cells to undergo lysis in presence of bile salts. This test is commonly performed in diagnostic laboratories for confirmation of pneumococcal isolates. It is the process in … Read more

Leucine aminopeptidase (LAP) Test – Principle, Procedure, Results

Leucine aminopeptidase (LAP) Test Principle, Procedure, Results

Leucine aminopeptidase (LAP) is an enzyme used to distinguish catalase-negative, gram-positive cocci of Aerococcus and Leuconostoc (LAP test negative) from other organisms in the group, such as Streptococcus, Enterococcus, Lactococcus, and Pediococcus (almost always positive).

Lipid Hydrolysis Test – Principle, Procedure, Result

Lipid Hydrolysis Test Principle, Procedure, Result

It is a biochemical test that is used to determine the ability of microorganism to produce and secrete the enzyme lipase. It is also referred to as lipase test. The complex lipids such as triglycerides are large molecules and these cannot pass through the bacterial cell membrane. Due to this reason some bacteria releases the … Read more

Replicative Transposition of DNA Transposons and Retrotransposons

Replicative Transposition of DNA Transposons and Retrotransposons

What is replicative transposition?  Replicative transposition is a type of transposition in which the transposable element is duplicated during its movement from one site to another. It is the process where the original transposable element remains at the donor site and a new copy is inserted into the target DNA. This is referred to as … Read more

Euglena – Definition, Characteristics, Structure, Reproduction, Importance

Euglena - Definition, Characteristics, Structure, Reproduction, Importance

What is Euglena? Euglena is a unicellular microscopic organism which belongs to the group of protists and it shows the characteristics of both plant and animal. It is generally found in fresh water bodies like ponds lakes and marshy places. It is a eukaryotic organism and the cell contains nucleus and other membrane bound organelles. … Read more

Cyclops – Habitat, Morphology, Life Cycle, Control, Importance

Cyclops

What is Cyclops? Cyclops is a genus of small freshwater crustaceans which belongs to the subclass Copepoda. It is commonly referred to as water flea due to its small size and free swimming nature. It is named after the one-eyed giants of Greek mythology as a single median eye is present at the centre of … Read more

Ouchterlony Double Immunodiffusion Method

Ouchterlony Double Immunodiffusion Method

Ouchterlony Double Immunodiffusion Method is a qualitative immunological technique that is used to study antigen–antibody reaction in vitro. It is the process in which both antigen and antibody are allowed to diffuse freely in a semi-solid medium usually agar or agarose gel. This method was developed by Örjan Ouchterlony and it is commonly used for … Read more