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Phenylalanine Deaminase Test – Principle, Procedure, Result

Phenylalanine Deaminase Test Principle, Procedure, Result

The phenylalanine deaminase test is a biochemical test used to identify bacteria that can deaminate the amino acid phenylalanine. It is the process where certain organisms convert phenylalanine into phenylpyruvic acid by removing the amine group. This is referred to as oxidative deamination and it is one of the important reactions for separating the PPM … Read more

Bacitracin Susceptibility Test – Principle, Procedure, Result

Bacitracin Susceptibility Test Principle, Procedure, Result

The Bacitracin susceptibility test is one of the antimicrobial disc tests used to distinguish between group A and other -hemolytic Streptococci.

Optochin Susceptibility Test – Principle, Objective, Procedure And Results

Optochin Susceptibility Test - Principle, Objective, Procedure And Results

Streptococcus pneumoniae can be distinguished from other viridans streptococci by showing that it is sensitive to optochin, dissolves in bile, reacts with a specific DNA probe, or has species-specific capsular polysaccharides. 

SXT (Sulfamethoxazole-Trimethoprim) Test – Principle, Procedure, Result

SXT Test - Principle, Procedure, Result

The SXT (sulfamethoxazole-trimethoprim) susceptibility test tells the difference between group A and B streptococci and other hemolytic streptococci. 

Heat Coagulation Test of Proteins – Principles, Procedure, Result

Heat Coagulation Test of Proteins - Principles, Procedure, Result

agulation of proteins is a test in biochemistry to find whether there are proteins such as albumin and globulin found in proteins. The coagulation of proteins in reaction the heat stimulus is an typical phenomenon. The process of coagulation by heat of proteins happens in two phases; denaturation or Agglutination, or the dissociation of the protein denatured in the form it is in.

Millon’s Test -Principle, Procedure, Result, Uses

Millon’s Test Definition, Principle, Procedure, Result, Uses

illon’s test can be described as an analytical test for the identification that the amino acid tyrosine which is the sole amino acid with the phenolic group.

Bial’s Test- Principle, Procedure, Result, Uses

Bial test for pentoses Principle, Objective, Procedure, Result

Bial test is a biochemical test which is used for the detection of pentose sugars and to differentiate them from hexose sugars. It is mainly employed for identifying sugars like ribose and xylose and is also useful for the detection of RNA because RNA contains ribose sugar. This test is based on the reaction of … Read more

Beta (β) Lactamase Test – Principle, Procedure, Results

Beta (β) Lactamase Test Principle, Procedure, Results

Many bacteria produce a group of enzymes known as beta-lactamases that are mediated through genes in plasmids or the chromosomes. Beta-lactamase can be a constant process or it may be triggered through contact with antimicrobials. Beta-lactamases hydrolyze (and consequently inhibit) the beta-lactam ring of many Cephalosporins as well as penicillins. Beta-Lactamase Test is a rapid test to determine the presence of beta-lactamase , an enzyme that is produced by the strains from Staphylococcus aureus Neisseria gonorrhoeae, Branhamella catarrhalis as well as Haemophilus influenzae. 

Nitrocefin Test (Beta-Lactamase Test) – Principle, Procedure, Uses

Nitrocefin test - Principle, Procedure, Uses

The nitrocefin biochemical test is a sensitive technique for detecting beta-lactamase-producing strains of N. gonorrhoeae, H. influenzae, Staphylococcus spp, Enterococcus spp, and Moraxella (Branhamella) catarrhalis. Nitrocefin is the only reliable test for detecting beta-lactamase-producing Enterococcus spp.

Mucic acid test – Principle, Procedure, Result, Uses

Mucic acid test - Principle, Procedure, Result

Mucic acid test is a specific chemical test used for the identification of galactose and galactose containing sugars like lactose. It is also referred to as galactaric acid test. It is based on the oxidation reaction of sugars with concentrated nitric acid. In this test the sugar sample is heated with concentrated nitric acid which … Read more

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