Decarboxylase Test – Principle, Procedure, Results, Uses
Decarboxylase Test Medium base is used to distinguish bacteria based on their ability to decarboxylate amino acids.
Sourav Pan
Hopkin’s Cole Test – Principle, Procedure, Result, Uses
It is a specific colour reaction used for the detection of amino acid tryptophan present in proteins. This test is also referred to as Adamkiewicz–Hopkins reaction or glyoxylic acid test. It is based on the presence of indole ring which is a characteristic structure of tryptophan and not found in other amino acids. Hence the … Read more
X and V factor Test – Principle, Purpose, Procedure, Result
Some bacteria make substances that other bacteria need to grow. The X factor and the V factor are two such substances. The heat-stable X-factor can be protoporphyrin IX, hemin, or other iron-containing porphyrins. It can be found in red blood cells and outside of them in the blood. The heat-sensitive V-factor may be nicotinamide adenine dinucleotide (NAD) or nicotinamide adenine dinucleotide phosphate (NADP), and it is mostly found inside red blood cells. The X and V factor test or a disc test can be used to find out if a bacterium depends on its X factor or its V factor. The results can help identify the bacteria.
MUG Test – Principle, Procedure, Result, Uses
The 4-Methylumbelliferyl-β-D-Glucuronide (MUG) test is a rapid diagnostic method used in microbiology for the detection of Escherichia coli in water food and environmental samples. It is based on the detection of β-glucuronidase enzyme activity which is produced by majority of E. coli strains. It is the process in which a specific fluorogenic substrate is utilised … Read more
Nelson-Somogyi method – Principle, Procedure, Result, Uses
Nelson–Somogyi method is a classical colorimetric method used for the quantitative determination of reducing sugars such as glucose and fructose. It is a highly sensitive method and is commonly used in biochemical and carbohydrate analysis. It is considered as a standard method because the estimation is based on stoichiometric reduction reaction and gives accurate measurement … Read more
Peroxide Value Test – Principle, Procedure, Result
The Peroxide Value test is the process used to estimate the extent of primary oxidation in fats and oils. It is the measure of hydroperoxides formed at the initial stage of lipid oxidation, and it is expressed as milliequivalent of active oxygen per kilogram of oil. It is the major indicator of freshness because these … Read more
Ninhydrin Test – Principle, Procedure, Result, Uses
What is the Ninhydrin Test? It is the chemical test used for detecting amino acids and other compounds having free amino groups. It is the process in which ninhydrin (2,2-dihydroxyindane-1,3-dione) reacts with amino acids and the reaction is accompanied by oxidative deamination. In this step, the amino group is removed and there is release of … Read more
Phenylalanine Deaminase Test – Principle, Procedure, Result
The phenylalanine deaminase test is a biochemical test used to identify bacteria that can deaminate the amino acid phenylalanine. It is the process where certain organisms convert phenylalanine into phenylpyruvic acid by removing the amine group. This is referred to as oxidative deamination and it is one of the important reactions for separating the PPM … Read more
Bacitracin Susceptibility Test – Principle, Procedure, Result
The Bacitracin susceptibility test is one of the antimicrobial disc tests used to distinguish between group A and other -hemolytic Streptococci.
Optochin Susceptibility Test – Principle, Objective, Procedure And Results
Streptococcus pneumoniae can be distinguished from other viridans streptococci by showing that it is sensitive to optochin, dissolves in bile, reacts with a specific DNA probe, or has species-specific capsular polysaccharides.