Subculturing technique for Broth Culture

Subculturing technique for Broth Culture

Aim Transfer of microbial cells from a parent culture medium to a fresh broth culture. Principle The broth is a liquid medium, which is used to grown microorganisms within the laboratory on a small scale. It also used in an industrial fermentor on a large scale. The media called broths, milks, or nutrient solutions are … Read more

Streak Plate Method – Patterns, Procedure, Principle

Streak Plate Method: Patterns, Procedure, Principle

Streak Plate Method Overview There are two types of microbial culture such as mixed culture and pure culture. The mixed culture contains two or more species while the pure culture contains only a single species.  During the identification of a microorganism, the first and important step is to isolate the individual species from a mixed … Read more

Fructose Metabolism Pathway – Fructolysis

Fructose Metabolism Pathway - Fructolysis

Location of Fructolysis Why does fructose metabolism or fructolysis occur in liver cells? Properties and Sources of Fructose Absorption of Fructose From Fructose to Fat/Fructolysis/fructose metabolism pathway Steps of fructose metabolism pathway 1. Step 1 2. Step 2 3. Step 3A Metabolic Pathway Notes: At this point, the liver cell can use the same steps … Read more

Difference between Photosystem 1 (PS1) and Photosystem 2 (PS2)

Difference between Photosystem 1 (PS1) and Photosystem 2 (PS2)

What is Photosystem 1 (PS1)? What is Photosystem 2 (PS2)? Difference between Photosystem 1 (PS1) and Photosystem 2 (PS2) Feature Photosystem I (PSI) Photosystem II (PSII) Location in Thylakoid Membrane Outer surface Inner surface Photocenter P700 P680 Absorbing Wavelength >680 nm <680 nm Photophosphorylation Both cyclic and non-cyclic Only non-cyclic Photolysis of Water No Yes … Read more

Top 15 Books for Molecular Biology

Top 15 Books for Molecular Biology

In this article, I will share a list of Top and Best Molecular Biology Books. In this paper on microbiology, you will study about Structures of DNA and RNA / Genetic Material,  Replication of DNA (Prokaryotes and Eukaryotes), Transcription in Prokaryotes and Eukaryotes, Post-Transcriptional Processing, Translation (Prokaryotes and Eukaryotes), Regulation of gene Expression in Prokaryotes … Read more

Total White Blood Cell (WBC) Count – Total Leucocyte Count (TLC)

Total White Blood Cell (WBC) Count - Total Leucocyte Count (TLC)

The White Blood Cells (WBCs) also known as Leucocytes are round nucleated cells in blood, that provide protection against foreign invaders. There are many types of White Blood cells found in blood, and identified as Granulocytes (Neutrophils, Eosinophils & Basophils) and Agranulocytes (Monocytes and Lymphocytes). The size of White Blood Cells can vary between 10 … Read more

Centrifuge Blood Sample – Purpose, Steps, Techniques

Centrifuge Blood Sample - Purpose, Steps, Techniques

What are the components of blood? Blood is a fluid tissue that circulates throughout the body and performs a number of important functions. It is made up of several components, including cells and plasma. Cells: Plasma: Plasma is the liquid portion of the blood that carries the cells and other substances throughout the body. It … Read more

Reticulocyte Count – Principle, Procedure, Calculations and Clinical Significances

Reticulocyte Count : Principle, Procedure, Calculations and Clinical Significances

What is Reticulocytes? Reticulocytes are immature red blood cells (erythrocytes) that have not yet completed the final stages of maturation. They are usually produced in the bone marrow and have a characteristic reticulated (network-like) appearance when stained with special dyes. Reticulocytes are important in the evaluation of anemia, as they provide information about the bone … Read more

Total Red Blood Cell (RBC) Count – Procedure, Principle, result

Total Red Blood Cell (RBC) Count - Procedure, Principle, Result

Haemocytometry is a quantitative method that quantifies the RBC count in blood samples. The manual method of counting RBCs is the Haemocytometer (or Neubauer’s chamber slides). Modern devices, such as photometric and electrometric counters, can be more precise or automated. They can count the cells in the blood sample. It is impossible to count the … Read more

Sahli’s Method For The Estimation Of Hemoglobin

Sahli’s Method For The Estimation Of Hemoglobin

Hemoglobin, often abbreviated as Hb, is a vital protein found in red blood cells (RBCs). Its primary role in the human body includes the transport of oxygen from the lungs to various tissues and the transfer of carbon dioxide from tissues back to the lungs. Additionally, hemoglobin plays a significant role in maintaining the pH … Read more