Salt Meat Broth
Salt Meat Broth is used as an enrichment medium for staphylococci isolation from severely contaminated specimens.
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Tryptone Soya Broth for the enrichment of Staph. aureus
Supplemented with sodium pyruvate and sodium chloride, tryptone soya soup can be used for selective enrichment of Staphylococcus Aureus.
Lysogeny broth (LB) Preparation
LB is the most widely used bacterial culture medium but its roots lie in the area of genetics of bacteriophage. Guiseppi Bertani developed the LB recipe when he was trying to increase the amount of plaque that formed on an indicator strain of Shigella (Bertani 1952). As per Bertani, LB has been often misinterpreted to mean “Luria Broth”, “Luria-Bertani” medium, or “Lennox Broth”; however the original acronym stood as “Lysogeny Broth” (Bertani, 2004). The agar version of the medium is identified as LA however, it is frequently called LB. While initially developed to study bacteriophage in addition to Shigella growth, LB subsequently became the most preferred medium to grow Escherichia bacteria and other species of the enteric.
Preparation of Solid Media – Agar deep tubes, Agar Slants, Plates
If the broth medium is supplemented with agar-agar it is referred to as agar medium like the nutrient Agar Medium (NAM) for the cultivation of bacteria potato dextrose (PDA) medium for the cultivation and storage of general fungi Czapek Dox agar (CDA) medium for fungi, starch-casein (SCA) media for the cultivation of actinomycetes, and so on.
Ashdown’s Agar – Composition, Principle, Preparation, Results, Uses
Ashdown’s medium can be described as a selective culture medium that allows for the identification and characterisation of Burkholderia pseudomallei (the bacteria which causes the disease melioidosis). The Ashdown’s Medium was initially identified by LR Ashdown in 1979.
Bird Seed Agar (Staibs Medium) – Composition, Principle, Preparation
Bird Seed Agar Bird Seed Agar is an effective solid medium to isolate selectively and differentially of Cryptococcus Neoformans from clinical specimens. It is utilized for the specific isolation from Cryptococcus neoformans as well as C. Gattii. C. Neoformans typically grows as yeast (unicellular) and reproduces via budding. Cryptococcus Neoformans is an enclosed yeast, which makes the enzyme phenoloxidase an enzyme that is essential to melanin production.
Preparation of Liquid Medium/broth
The process of growing an organism in the surface of a medium is known as culture. The food source that supports the development of the organism is known as a culture medium. The media for culture are designed so that the organism will receive all the nutrition requirements. However, the media for culture are made in laboratories by weighing and dispensing particular ingredients or by purchasing ready-made media on the market. The majority of the media include organic as well as inorganic nutrients. However, to cultivate a variety of microorganisms, special media are created. If the media is to be solidified required, agar-agar can be mixed with other ingredients. The culture media can be classified into three categories that include semi-synthetic, natural and synthetic media each of which is employed for microbiological studies.
VRBA(Violet Red Bile Agar) – Composition, Principle, Preparation, Results, Uses
Coliforms are all facultative and aerobic rod-shaped, gram-negative, non-spore-forming bacteria that produce lactose by fermentation with gas and acid production within 48 hours, at temperatures of 35 degrees Celsius. Methods to identify, enumerate and presumedly identify coliforms are utilized in the testing of dairy and food products. One method of performing the presumptive test to identify coliforms employs the Violet Red Bile Agar (VRBA). The Violet Red Bile Aggar (VRBA) is an selec-tive medium that can be used to detect and quantify lactose-fermenting coliform microorganisms.
Tryptic Soy Agar (TSA) – Composition, Principle, Preparation, Results, Uses
Tryptic Soy Agar can be used to grow microorganisms. These media are non-selective and general-purpose. They provide enough nutrients to support a variety of microorganisms. This medium can also be used to grow, store, maintain, and transport pure microorganism cultures.
Baird Parker Agar – Composition, Principle, Preparation, Results, Uses
Baird Parker Agar was created by Baird Parker using the Tellurite–glycine formulation of Zebovitz et al. It allows for a good differentiation between coagulase positive strains.