Nuclear Fast Red Solution Preparation and Application

Buffer preparation

This dye has been used as a counterstain in saturated aqueous potassium alum and in a counterstain solution of 0.1% (w/v) with 5% aluminum sulfate for a Prussian Blue Stain for ferric iron. It has been used as a reagent for calcium in tissue where it forms a scarlet lake.

5-Fluorocytosine (5-FC) Solution Preparation

5-Fluorocytosine (5-FC) Solution Preparation

5-fluorocytosine (5-FC) also known as Flucytosine. 5-Fluorocytosinewas first synthesized in 1957 as anti-tumor drug. It is an antifungal medication which is used with amphotericin B against Candida infections and cryptococcosis. This drug is mainly taken by mouth and by injection into a vein.

Lysine Iron Agar (LIA) Test Principle, Procedure, Result

Lysine Iron Agar (LIA) Test Principle, Procedure, Result

What is the Lysine Iron Agar (LIA) Test? The solid medium Lysine Iron Agar (LIA) is indicated for use in qualitative techniques for the differentiation of microorganisms based on the generation of lysine decarboxylase and hydrogen sulphide. Purpose of Lysine Iron Agar (LIA) Test Principle of Lysine Iron Agar (LIA) Test Lysine Iron Agar Composition … Read more

Stokes Disc Diffusion Method – Principle, Procedure, Result.

Stokes Disc Diffusion Method Principle, Procedure, Result.

Stokes disc diffusion method isn’t as well-standardized as Kirby-Bauer’s method and is utilized in labs especially where the exact amount of antimicrobial present in discs isn’t known because of the difficulty in getting discs and correctly storing them or when other requirements needed for the Kirby-Bauer method cannot be fulfilled.