Nagler Reaction – Lecithinase Test

Nagler Reaction - Lecithinase Test

Nagler’s Reaction or Lecithinase test is a test in biochemistry used to detect organisms that produce the phospholipases (lecithinases) e.g. Clostridium perfringens. Its alpha (a) toxin from C. perfringens exhibits the phospholipase enzyme activity, which aids in the distinction between C. perfringens and the other Clostridium species that generate the enzyme lecithinase (C.baratti, C.absonum, C.bifermantans, C.sordelli, and C.novyi) through neutralization of lecithin C activity with an antitoxin.

Widal Test – Principle, Procedure, Result, Advantages, Limitation

Widal Test Principle, Procedure, Result, Advantages, Limitation

The Widal test is an agglutination test that determines any antibodies present in the patient’s serum that are generated against the causal agents of the enteric febrile illness (Salmonella Typhi, and Salmonella paratyphi B, A as well as C).

Types of Blood Collection Tubes and Their uses

Types of Blood Collection Tubes and Their uses

Evacuated Tubes that are used for blood collection for different laboratory tests are made up of tubes in variable sizes, and color-coded tops to indicate tube contents. The majority of blood collection tubes contain an ingredient that either speeds up the blood’s clotting (clot activator) or stops blood from becoming clot-free (anticoagulant). The following list lists the most frequently utilized blood collection tubes, along with their additives, and their uses in lab: