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Robertson’s Cooked Meat Medium (RCM Medium) – Composition, Preparation, Uses

Robertson’s Cooked Meat Medium (RCM Medium) Preparation, Composition.

Cooked Meat Medium was originally developed by Robertson to cultivate specific anaerobes isolated from wounds.
The current formulation is a modified version, sometimes referred to by the name of Chopped Meat Medium, which allows for the growth of a variety of spores and non-spore-forming strict anaerobes.

Nutrient Agar – Principle, Composition, Preparation, Results, Uses

Nutrient Agar - Principle, Composition, Preparation, Results, Uses

Nutrient agar is an essential culture medium that is commonly used for the cultivation of non-fastidious microorganisms. It can also be used to ensure quality control and quality prior to serological or biochemical tests.

Viral Transport Media (VTM) – Definition, Principle, Preparation, Application

Viral Transport Media (VTM) Definition, Principle, Preparation, Application

A viral transport medium (VTM) is an essential nutrient that is used to transport and sustain the viability of samples (viruses) to an laboratory for analysis and identification of the specimen.

Decarboxylase Broth Protocol

Decarboxylase Broth Protocol

Moeller introduced the decarboxylase media to detect the ability of bacteria to decarboxylate amino acid. They were used for the first time to detect lysine, ornithine, and arginine decarboxylases among Enterobacteriaceae bacteria.

Columbia CNA Agar – Composition, Preparation, Uses, and Results

Columbia CNA Agar - Composition, Preparation, Uses, and Results

What is Columbia CNA Agar? Columbia CNA Agar with 5% Sheep Blood Composition of Columbia CNA Agar per Liter of Medium The composition of Columbia CNA Agar can be summarized as follows, considering the information provided: Ingredients: The base of Columbia CNA Agar is prepared using a nutritionally rich medium that includes different peptones to … Read more

Bile Esculin Agar (BEA) – Principle, Composition, Preparation, Results, Uses

Bile Esculin Agar (BEA) - Principle, Composition, Preparation, Results, Uses

What is Bile Esculin Agar (BEA)? Bile Esculin Agar (BEA) Composition Bile Esculin Agar (BEA) contains the following ingredients per liter of the medium: The final pH of the medium, when measured at 25°C, is approximately 6.6±0.2. These specific components and their concentrations are carefully selected to create a medium that is selective and differential … Read more

Columbia Blood Agar Base w/ Hemin – Composition, Preparation, Principle, Result, Uses

Columbia Blood Agar Base w/ Hemin - Composition, Preparation, Principle, Result, Uses

What is Columbia Blood Agar Base? Principle of Columbia Blood Agar Base The principle of Columbia Blood Agar Base lies in its composition and functionality in supporting the growth and differentiation of various bacterial species. Here are the key principles of Columbia Blood Agar Base: Composition of Columbia Blood Agar Base Ingredients Gms/Litre Peptone special … Read more

Simmons Citrate Agar – Composition, Principle, Preparation, Results, Uses

Simmons Citrate Agar - Composition, Principle, Preparation, Results, Uses

What is Simmons Citrate Agar? Composition of Simmons Citrate Agar Ingredients Gms/liter Magnesium sulfate 0.200 Ammonium dihydrogen phosphate 1.000 Dipotassium phosphate 1.000 Sodium citrate 2.000 Sodium chloride 5.000 Bromothymol blue 0.080 Agar 15.000 Final pH ( at 25°C) 6.8±0.2 Principle of Simmons Citrate Agar The principle of Simmons Citrate Agar revolves around the ability of … Read more

Transport Media – Definition, Types, Principle, Uses, Examples

Transport Media Used in Laboratories

Transport media are basically buffer solutions that contain carbohydrate, peptones, along with other nutritional elements (excluding growing factors) that are designed to protect the health of bacteria during transport, without allowing for their multiplicity. The main goal of utilization of the medium is to keep the sample as close to its original condition as is possible.

Burkholderia Cepacia Agar Base – Preparation, Composition, Principle

Burkholderia Cepacia Agar Base Preparation, Composition, Principle

Burkholderia cepacia can be viewed as an opportunity-based bacterium that is associated with nosocomial illnesses caused by contamination of medical equipment and disinfectants. However, the highest risk group is CF patients. People with cystic fibrosis are at an increased risk of infection. those with the infection, if not treated are prone to rapid declines of lung functions, frequent bacteremia and even death due to lung dysfunction.

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