BNO Team
What is Simmons Citrate Agar? Composition of Simmons Citrate Agar Ingredients Gms/liter Magnesium sulfate 0.200 Ammonium dihydrogen phosphate 1.000 Dipotassium phosphate 1.000 Sodium citrate 2.000 Sodium chloride 5.000 Bromothymol blue 0.080 Agar 15.000 Final pH ( at 25°C) 6.8±0.2 Principle of Simmons Citrate Agar The principle of Simmons Citrate Agar revolves around the ability of … Read more
Transport media are basically buffer solutions that contain carbohydrate, peptones, along with other nutritional elements (excluding growing factors) that are designed to protect the health of bacteria during transport, without allowing for their multiplicity. The main goal of utilization of the medium is to keep the sample as close to its original condition as is possible.
Burkholderia cepacia can be viewed as an opportunity-based bacterium that is associated with nosocomial illnesses caused by contamination of medical equipment and disinfectants. However, the highest risk group is CF patients. People with cystic fibrosis are at an increased risk of infection. those with the infection, if not treated are prone to rapid declines of lung functions, frequent bacteremia and even death due to lung dysfunction.
Salt Meat Broth is used as an enrichment medium for staphylococci isolation from severely contaminated specimens.
Supplemented with sodium pyruvate and sodium chloride, tryptone soya soup can be used for selective enrichment of Staphylococcus Aureus.Â
LB is the most widely used bacterial culture medium but its roots lie in the area of genetics of bacteriophage. Guiseppi Bertani developed the LB recipe when he was trying to increase the amount of plaque that formed on an indicator strain of Shigella (Bertani 1952). As per Bertani, LB has been often misinterpreted to mean “Luria Broth”, “Luria-Bertani” medium, or “Lennox Broth”; however the original acronym stood as “Lysogeny Broth” (Bertani, 2004). The agar version of the medium is identified as LA however, it is frequently called LB. While initially developed to study bacteriophage in addition to Shigella growth, LB subsequently became the most preferred medium to grow Escherichia bacteria and other species of the enteric.
If the broth medium is supplemented with agar-agar it is referred to as agar medium like the nutrient Agar Medium (NAM) for the cultivation of bacteria potato dextrose (PDA) medium for the cultivation and storage of general fungi Czapek Dox agar (CDA) medium for fungi, starch-casein (SCA) media for the cultivation of actinomycetes, and so on.
Ashdown’s medium can be described as a selective culture medium that allows for the identification and characterisation of Burkholderia pseudomallei (the bacteria which causes the disease melioidosis). The Ashdown’s Medium was initially identified by LR Ashdown in 1979.
Bird Seed Agar Bird Seed Agar is an effective solid medium to isolate selectively and differentially of Cryptococcus Neoformans from clinical specimens. It is utilized for the specific isolation from Cryptococcus neoformans as well as C. Gattii. C. Neoformans typically grows as yeast (unicellular) and reproduces via budding. Cryptococcus Neoformans is an enclosed yeast, which makes the enzyme phenoloxidase an enzyme that is essential to melanin production.Â
The process of growing an organism in the surface of a medium is known as culture. The food source that supports the development of the organism is known as a culture medium. The media for culture are designed so that the organism will receive all the nutrition requirements. However, the media for culture are made in laboratories by weighing and dispensing particular ingredients or by purchasing ready-made media on the market. The majority of the media include organic as well as inorganic nutrients. However, to cultivate a variety of microorganisms, special media are created. If the media is to be solidified required, agar-agar can be mixed with other ingredients. The culture media can be classified into three categories that include semi-synthetic, natural and synthetic media each of which is employed for microbiological studies.
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