Types of Staining Techniques
Staining is a method used to boost the contrast of samples, usually at the microscopic scale.
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Types of Stains used in Microbiology
Different stains react or concentrate on different areas of a tissue or cell These properties can be utilized to highlight certain areas or regions. A few of the most well-known biological staining methods can be found below. If not otherwise indicated All of these dyes can be used on tissues and cells that are fixed as well as essential dyes (suitable for use in live organisms) are indicated.
Albert Stain – Principle, Procedure, Result
Different stains have been developed over time to distinguish bacteria species, separating them morphologically and the specific characteristics they possess. The most popular stain is Gram staining, acid-fast staining, and endospore staining. Each stain aims at identifying and defining bacteria according to their forms and morphologies.
Papanicolaou Staining (Pap stain) – Principle, Procedure, Results, Applications
Papanicolaou stain can also be referred to as the pap stain, and the process that causes the stain is referred to as a pap smear.
Nuclear Staining by Giemsa stain – Procedure, Principle, Result
Bacterial cells contain nuclear material, made of single-stranded circular DNA, in contrast to eukaryotes. This genetic material is present within the membrane-bounded structure known as the nucleus. In case of prokaryotes the nuclear material is present within the nucleoid which lacks the nuclear membrane and does not follow the mitosis or miosis cell division. Some … Read more
Viability Staining Method For Bacteria
Aim To distinguish between living and dead cells. Principle The viability Staining Method mainly used to detect living and dead cells in culture. This method is based on the fact that, certain changes take place in dead cells which result in these cells giving a different reaction to stains from that of living cells. Requirement … Read more
Flow Cytometry Laboratory for cell viability staining using propidium iodide.
Flow cytometry is a fast and stable method for the quantification of viable cells. Determining cell viability is a significant step when assessing a cell’s reply to medication or other environmental agents. It is additionally essential to recognize dead cells in a cell suspension in order to eliminate them from the study. Dead cells can … Read more
Nuclear Staining of Filamentous Fungi
Fungi are eukaryotic and contain well-organized nuclei. The fungal nucleus is membrane bound and consist of two membranes of characteristic pores, a nucleolus and chromatin strand that becomes organized into chromosome during nuclear division. The fungal nuclei are extremely minute, therefore study with the light microscope is very difficult. The cells of fungal hypae are … Read more
Cell Wall Staining by Dayr’s Method
The composition of cell wall varies from species to species, it has been reported that the main constituents of cell wall is chitin, hemicellulose, and cellulose. In addition, prokaryotic cell wall contains peptidoglycan (also known as murein and mucopeptide). Peptidoglycan is mainly composed of sugar, amino acids (peptide; amino acids + glycan; sugar).
Cell Membrane Staining Procedure, Principle, Result
The cell membrane is a biological membrane which also termed a plasma membrane (PM) or cytoplasmic membrane. It divides the interior of the cell from the outside environment, thus protects the cell from environmental stress. The cell membrane is a lipid bilayer, contains cholesterols between phospholipids which maintain their fluidity at various temperatures. The membrane … Read more