Downstream Processing Steps

Downstream Processing Steps

What is Downstream processing? Downstream processing refers to the procedures required to recover and purify fermentation-produced products. In the step of downstream processing, particles, mostly cells, are separated from the liquid using filtration, centrifugation, and flocculation. Steps Of Downstream Processing Five stages in downstream processing after Fermentation: Stage 1: Solid-Liquid Separation 1. Flotation 2. Flocculation … Read more

Citric Acid Production

Citric Acid Production

What is Citric Acid? Properties of Citric Acid – C6H8O7 C6H8O7 Citric Acid Molecular Weight/ Molar Mass 192.124 g/mol Density 1.66 g/cm³ Boiling Point 310 °C Melting Point 153 °C Citric Acid structure – C6H8O7 Which Microorganism is used for Citric Acid Production? Numerous microorganisms are utilised in the production of citric acid. There are … Read more

Lactic Acid Production

Lactic Acid Production

Lactic acid is manufactured commercially by both synthetic and fermentation processes. It is made synthetically in Japan and the United States. European manufacturers, on the other hand, make lactic acid by fermentation. European producers account for between 28,000 and 30,000 tonnes per year of the world’s estimated lactic acid production capacity. Pharmaceutical industries (e.g. iron … Read more

Photo bioreactor – Definition, Types, Application, Advantages

Photo bioreactor Definition, Types, Application, Advantages

Photo bioreactor is an innovative type of reactor that has the advantages of biofilm reactors and photobioreactors that can be utilized to cultivate microalgae in wastewater treatment facilities (WWTPs). The major benefit of this type of system is its capacity to use sunlight as a source of energy for photosynthesis, which reduces the requirement for carbon from external sources.

Production Strain – Screening, Development, Preservation, Preparation

Production Strain - Screening, Development, Preservation, Preparation

Sources of industrially significant microorganisms and methods for isolating, preserving, and maintaining them, as well as strain improvement, A production strain is the most crucial factor for the success of any fermentation enterprise. Utilizing a producing strain with the following four traits is extremely desirable: 1. Screening Techniques Screening refers to the detection and isolation … Read more

Bioprocess Engineering – Definition, Types, Uses

Bioprocess Engineering Definition, Types, Uses

Bioprocess engineering involves the design of, operation, management, and optimizing biochemical processes that involve different biological pathways, or reactions that are mediated through living cells from plants, animals and microorganisms as well as enzymes in controlled conditions to facilitate the efficient biotransformation process of raw materials into a variety of goods at the appropriate scale.

Radial Immunodiffusion – Principle, Procedure, Result, Uses

Radial Immunodiffusion Principle, Procedure, Result

Single Radial Immunodiffusion also referred to as Mancini technique is a quantitative immunodiffusion technique that is used to measure the antigen’s concentration by measuring the size of the precipitin-based ring created by the interaction between the antigen with the antibody at the optimal concentration. The antibody is embedded in the agarose gel, while the antigen is diffused into it in the form of a radiatal pattern.

Rapid Plasma Reagin (RPR) Test – Principle, Procedure, Result, Applications

Rapid Plasma Reagin (RPR) Test - Principle, Procedure, Result, Applications

What is Rapid Plasma Reagin (RPR) Test? Principle of Rapid Plasma Reagin (RPR) Test The principle of the Rapid Plasma Reagin (RPR) test is based on the detection of antibodies produced in response to antigens released by damaged host cells during a syphilis infection. The RPR test is a macroscopic, non-treponemal flocculation card test that … Read more

Mantoux Test – Procedure, Normal Range and Result Interpretation

Mantoux Test - Procedure, Normal Range and Result Interpretation

What is Mantoux Test? Principle of Mantoux Test The Mantoux test operates on the principle of delayed-type hypersensitivity, specifically Type IV hypersensitivity. This test is designed to assess a person’s cell-mediated immunity against the bacterium Mycobacterium tuberculosis. To conduct this test, a substance known as Purified Protein Derivative (PPD) is used, which is essentially a … Read more

Neutralization Test – Definition, Principle, Types, Procedure, Results, Applications

Neutralization Test

What is Neutralization Test? Principle of Neutralization  The neutralization principle is based on the understanding that certain antibodies or antitoxins can reduce or neutralize the various biological effects caused by enzymes, toxins, and viruses. There are two main types of neutralization tests: In both types of neutralization tests, the goal is to assess the ability … Read more